Data management
Datasets:
Data underlying the chapter and publication: Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine Dehydrogenase

Title: Data underlying the chapter and publication: Synthesis of Chiral Amines via a Bi-Enzymatic Cascade Using an Ene-Reductase and Amine Dehydrogenase

Authors: Ewald P. J. Jongkind, Aurélie Fossey-Jouenne Ombeline Mayol Anne Zaparucha, Carine Vergne-Vaxelaire, and Caroline E. Paul
Link to publication: doi.org/10.1002/cctc.202101576

Short description: This dataset contains data collected during experiments at Delft University of Technology, as Chapter 2 of the dissertation written by Ewald Jongkind

Contact person: Caroline Paul, c.e.paul@tudelft.nl
Delft University of Technology – Faculty of Applied Sciences
Van der Maasweg 9 , 2629 HZ, Delft, The Netherlands

***Purpose of the test campaign***
The purpose of these experiments was to investigate combining two enzyme types, named OYE and AmDHs, into a bi-enzymatic cascade to synthesize chiral amines.

The data in this dataset was collected in the Biocatalysis Laboratory of the Delft University of Technology - Faculty of Applied Sciences, between September 2019 and September 2021.

**Test equipment***
Test equipment information could be found in the supplementary information of the chapter.

***Description of the data in this data set***
Corresponding context, methods and conditions could be found in the publication and supporting information of this work (see ChemCatChem).

Documents:

Ch1E01 SDS-PAGE.zip contains all gels identifying enzyme expression, descriptions are shown in supporting information

Ch2E02 UV spectra OYEs (determined by UV-VIS) concentrations of Old Yellow Enzymes determined by the flavin content, detectable by UV. data is shown from TsOYE, C25D/I67T-TsOYE, GkOYE, OYE2, GluER and Yqjm

Ch2E03 Activity assay (determined by UV-VIS) contains two documents:
2022-04-19 Specific activity each substrate and different reaction conditions: contains all activity assay data shown in manuscript, substrate labels can be found in supporting information
2021-03-24 Measured activities of substrates with TsOYE GkOYE and GluER with NAD(P)H: contains extra data shown in supporting information how OYEs act with NADH vs NADPH

Ch2E04 Reactions (determined by GC-FID): contains the following data:
Substrate conversions 1d 1e and 1f: data of substrates in different buffers
2021-11-01 conversions and DMSO impact: figures containing data on OYE activity in different buffers, with different cofactors, different substrates and in presence of increasing DMSO percentage
2021-07-06 trans-2-methylbutenal and trans-2-methylpentenal 1h reduction TsOYE GkOYE GluER
AmDH _ Screening: shows all reductive aminations of AmDHs used in this work
2021-07-06 cascade conversions into amine product: figure used to prepare overview of cascade results with ee and de values