README for Figure 1_HTkA

*** This file contains the raw data obtained from tethered particle motion experiments shown in Figure 1 of:
Article: Modulation of archaean hypernucleosome structure and stability by Mg2+
Authors: Ilias Zarguit, Marc K. M. Cajili, Bert van Erp, Samuel Schwab, Nico van der Vis, Marianne Bakker, John van Noort, and Remus T. Dame
Corresponding author: rtdame@chem.leidenuniv.nl

Legend Figure 1: T. kodakarensis and M. fervidus histones form hypernucleosomes upon binding DNA. TPM experiments show that (A) HTkA and HTkB and (B) HMfA and HMfB assemble into hypernucleosomes on DNA substrates in 25 mM Tris-HCl pH 7 and 75 mM KCl. Each measurement (N ≥ 100) was performed in duplicate, with the error bars representing the standard deviation. Dashed lines are added to guide the eye.

*** The data was obtained using tethered particle motion as described in the associated article.

*** Each individual sheet contains data obtained for each concentration of HTkA (as indicated in the sheet name). The sheet "Averaged Values" contains the average plotted in Figure 1.  
Each sheet contains data from individual tethered DNA molecules or protein-DNA complexes, with each row corresponding to one bead/tether.

Column A: Root mean square = RMS in nm
Column B: Anisotropy = Anisotropic ratio (movement in x-direction/movement in y-direction)
Column C: Stdev = Standard deviation
Column G: Fit parameters = The mean and standard deviation values obtained from fitting a normal distribution to the root mean square values (column A)

The values given in column B and C are tether quality criteria and used for data filtering as described in the associated article.
Histograms showing the distribution of the root mean square values are shown together with the fit and a kernel density estimator.
