README for SupplementaryFigure3_HMfA

*** This file contains the raw data obtained from tethered particle motion experiments shown in Supplementary Figure 3 of:
Article: Modulation of archaean hypernucleosome structure and stability by Mg2+
Authors: Ilias Zarguit, Marc K. M. Cajili, Bert van Erp, Samuel Schwab, Nico van der Vis, Marianne Bakker, John van Noort, and Remus T. Dame
Corresponding author: rtdame@chem.leidenuniv.nl

Legend Supplementary Figure 3:  Titration experiments with increasing MgCl2 at concentrations of (A) 40 nM HMfA and (B) 7.5 nM HMfB reveal an Mg2+ concentration-dependent increase of archaeal nucleosome formation

*** The data were obtained using tethered particle motion as described in the associated article.

*** Each individual sheet contains data obtained for each concentration of Mg2+ (as indicated in the sheet name). The sheet "Averaged Values" contains the average plotted in Supplementary Figure 3.  
Each sheet contains data from individual tethered DNA molecules or protein-DNA complexes, with each row corresponding to one bead/tether.

Column A: Root mean square = RMS in nm
Column B: Anisotropy = Anisotropic ratio (movement in x-direction/movement in y-direction)
Column C: Stdev = Standard deviation
Column G: Fit parameters = The mean and standard deviation values obtained from fitting a normal distribution to the root mean square values (column A)

The values given in column B and C are tether quality criteria and used for data filtering as described in the associated article.
Histograms showing the distribution of the root mean square values are shown together with the fit and a kernel density estimator.
