README for Supplementary Figure 5

*** This file contains the raw data obtained from magnetic tweezers experiments shown in Supplementary Figure 5 of:
Article: Modulation of archaean hypernucleosome structure and stability by Mg2+
Authors: Ilias Zarguit, Marc K. M. Cajili, Bert van Erp, Samuel Schwab, Nico van der Vis, Marianne Bakker, John van Noort, and Remus T. Dame
Corresponding author: rtdame@chem.leidenuniv.nl

Legend Supplementary Figure 5: A) A typical force-extension curve, where a HTkB hypernucleosome is being pulled (orange) and next the force is slowly released, which is reflected by the retraction curve (purple). No hysteresis is observed, indicating that the hypernucleosome complex is in thermodynamic equilibrium and that the histone dimers do not dissociate from the DNA. Hypernucleosomes that are not in thermodynamic equilibrium exhibit hysteresis and cannot be quantitatively analyzed using the model devised by Henneman et al. (2021) B) A typical force-extension curve of a HMfB hypernucleosome. The red line shows the model fitted to our data in blue. This allows us to extract quantitative values from our data. 
 
*** The data was obtained using magnetic tweezers as described in the associated article.

*** Columns show F (Force in pN) and z (Distance in micrometer) of HTkB hypernucleosomes during extension and retraction, as indicated.

