README for Figure 5A

*** This file contains the raw data obtained from magnetic tweezers experiments shown in Figure 5A of:
Article: Modulation of archaean hypernucleosome structure and stability by Mg2+
Authors: Ilias Zarguit, Marc K. M. Cajili, Bert van Erp, Samuel Schwab, Nico van der Vis, Marianne Bakker, John van Noort, and Remus T. Dame
Corresponding author: rtdame@chem.leidenuniv.nl

Legend Figure 5: Mg2+ does not affect the compactness of HMfB fibers. A) Force-extension curves and fits of HMfB hypernucleosome in the absence (gstack=3.64 kBT, gwrap=2.78 kBT, stiffness=0.81 pN/nm and angle=9.99°) and the presence of 2 mM Mg2+ (gstack=3.99 kBT,  gwrap=2.42 kBT, stiffness=0.78 pN/nm and angle=10.2°), as indicated. Unlike the HTk histones, we do not observe a noticeable difference between the traces, also reflected by MST experiments. B-F) The histograms of stacking energy, wrapping energy, stiffness, bending angle, and number of bound dimers of HTkB in the absence and the presence of Mg2+. The histograms were fitted with a normal distribution. The mean and standard error of mean of all fit parameters can be found in Table 2. 

*** The data was obtained using magnetic tweezers as described in the associated article.

*** Columns show F (Force in pN) and z (Distance in micrometer) of HMfB hypernucleosomes in the absence (No Mg) and presence of 2 mM Mg2+ (Mg). 