Column name	Explanation
#SampleID	Sample ID
LibraryNumber	"sequence library (1-3, is F-H)"
BarcodeNumber	"barcode primer set, 1-70, per sequence library"
ContainerID	container ID
Diet	"type of feed substrate: CF = chicken feed, CM = chicken manure"
Treatment	"Treatment. For larvae and substrates: S/E = control treatment, comprising untreated substrate and larvae from untreated eggs; Si/E = sterilized (autoclaved) substrate with 10% untreated substrate as inoculum (i) and larvae from untreated eggs; Si/Es = sterilized substrate with 10% untreated substrate as inoculum and larvae from sterilized (s, surface-disinfected) eggs; Ss/E = sterilized diet (s, without inoculum) and larvae from untreated eggs. For eggs: E = untreated eggs; Es = surface-disinfected eggs."
Timepoint	"sampled timepoint: day 0, 15, 22, or 30. Day 22 samples are from chicken feed LnDs; day 30 samples are from a pilot experiment (irrelevant to the study)."
Rep	"replicate within a treatment x timepoint x type combination: for chicken feed 1-4, for chicken manure 1-6"
Block	"batch of experiment (each batch was started in a different week): 1-5. Batches 1-2 are chicken feed treatments, batches 3-5 are chicken manure treatments. Each batch contained 2 replicates of a treatment within a diet."
Type	"sample type: larvae, substrate, eggs, isol_blank (no-template control of DNA isolation), pcr_blank (no-template control of PCR), mock (positive control, synthetic mock community)"
nCycles	number of cycles in the PCR program: 25 or 30
DNAconc	"DNA concentration of PCR product, in ng/ul"
Pilot	"Options: no (main analysis), no (sample of a pilot experiment, irrelevant to the study)"
Duplicate	"Options: no (main analysis), biological (DNA isolation replicate), technical (PCR replicate)"
Description	Sample ID
	
"""na"" or ""NA"" in a field stands for ""not applicable""."	
