Single-Molecule Localization Microscopy (SMLM) 2D Digits 123 and TOL letters datasets

1. Introductory information

The repository contains localization microscopy data (list of coordinates) of 1, 2 and 
3 Digits and a 3x4 grid (\Digits subdirectory) and T, O and L Letters (\Letters subdirectory)
DNA-origmai nanostructures imaged using PAINT technique.

The files are MATLAB .mat files that can be opened using any version of 
Mathworks MATLAB software.

For further questions you can contact:
Teun Huijben <tepet@dtu.dk>
Hamidreza Heydarian <H.Heydarian@tudelft.nl> or <hr.heidarian@gmail.com> and
Bernd Rieger <b.rieger@tudelft.nl> and
Sjoerd Stallinga <S.Stallinga@tudelft.nl>

2. Methodological information

All the information regarding data acquisition, collection and analysis can be
found on the following papers:
[1] Huijben T.*, Heydarian H.*, Auer A., Schueder F., Jungmann R. , Stallinga S, Rieger, B. 
Detecting structural heterogeneity in single-molecule localization microscopy data,
Nature Communications (2021).
[2] Alexander Auer, Maximilian T Strauss, Sebastian Strauss, Ralf Jungmann, nanoTRON:
a Picasso module for MLP-based classification of super-resolution data, Bioinformatics, 
Volume 36, Issue 11, June 2020, Pages 3620–3622, https://doi.org/10.1093/bioinformatics/btaa154 

3. Data specific information

Each dataset contains a cell array of particles. When loaded in MATLAB, you can
access each individual particle using the variable particles{1,index}. Each 
cell in this array is a MATLAB structure with two fields: points and sigma. The
first field, i.e. particles{1,index}.points is an Nx2 array containing N 
localization data (x and y) in camera pixel unit. The second field, i.e. 
particles{1,index}.sigma which is an Nx1 array contains the localization 
uncertainties in SQUARED camera pixel unit. The localization uncertainties are isotropic
in lateral direction therefore sigma_x = sigma_y.

Digits dataset contains data from experiments where the different structures were imaged separately 
in different experiments (Digits\Imaged_Separately) and imaged together (Digits\Imaged_Together), 
e.g. the structures were mixed and imaged in a single field-of-view. 

Similarly, Letters dataset contains data from experiments where T and O letter are imaged 
together (Letters\TO_Imaged_Together), T, O and L are imaged separately 
(Letters\TOL_Imaged_Separately) and T, O and L are imaged together (Letters\TOL_Imaged_Together). 
Details about the design of the DNA origami structures and experimental imaging settings can be 
found in [1] and [2], for respectively, the Letters and Digits dataset. 

4. Sharing and Access information

This dataset is made available under the Attribution-NonCommercial license 
CC BY-NC.






