********Title of the dataset:********
Dataset accompanying "Modelling anaerobic sulfide removal by sulfide shuttling bacteria"

********Creators:********
R Linssen
Rikke.linssen@wur.nl

********Contributors:********
Joris Bergman
Annemerel Mol
Annemiek ter Heijne
Karel Keesman (karel.keesman@wetsus.nl)

********Related publication:********
Modelling anaerobic sulfide removal by sulfide shuttling bacteria
https://doi.org/10.1016/j.biortech.2024.131064

********Description:********
In anaerobic conditions, haloalkaline sulphide oxidising bacteria (SOB) are able to spatially separate sulphide removal and oxygen consumption, thereby shuttling sulphide from sulphidic to aerobic environments. How SOB anaerobically remove sulphide is not yet fully understood. To further describe sulphide shuttling, anaerobic sulphide removal experiments were performed at different pH from 7.5 to 10. SOB were isolated from bioreactor effluent of a pilot-scale desulphurisation setup, resuspended in 1 M bicarbonate buffer, made anaerobic by flushing with nitrogen gas, and fed 0.35 mM sulphide. For half an hour, the sulphide concentration was measured periodically. Using this dataset, the related publication shows the use of mathematical models to describe anaerobic sulphide removal and its dependency on pH. 
********This dataset contains the following files:********
Data_NGS.xlsx – This file contains the relative abundances obtained using New Generation Sequencing (NGS) of the mixed sulphide oxidising culture used during the sulphide removal experiments. Whenever possible, the OTUs (identified in column A) are classified by Domain>Phylum>Class>Order>Family>Genus>Species in column B to H. The amount of measured copies per OTU is given in column I. 
Data_sulphide removal at pH.xlsx – This file contains sulphide concentration (mg/L) measured in time (minutes) during the sulphide removal experiments performed at different pH (7.5, 8, 9, 10). Experiments were performed in quadruplicate. 
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This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.
