********Title of the dataset:********
Dataset accompanying "Revealing cellular (poly)sulphide storage in electrochemically active sulphide oxidising bacteria using rotating disc electrodes "

********Creators:********
R Linssen
Rikke.linssen@wur.nl

********Contributors:********
Annemiek ter Heijne (Annemiek.terheine@wur.nl)
Sanne de Smit
Katharina Röhring (neé Neubert)
Falk Harnisch

********Related publication:********
Revealing cellular (poly)sulphide storage in electrochemically active sulphide oxidising bacteria using rotating disc electrodes
https://doi.org/10.1016/j.bioelechem.2024.108710 

********Description:********
In anaerobic conditions, sulphide oxidising bacteria (SOB) are able to spatially separate sulphide removal and electric current production. How SOB anaerobically remove sulphide, store charge and produce electric current is not yet clear. With the use of a rotating disc electrode, the redox behaviour of (poly)sulphide in absence and presence of SOB was investigated at haloalkaline conditions. Cyclic voltammetry (CV) was performed on different sulphur species in the presence or absence of SOB. Different rotation speeds and scan rates were tested. The full methodology can be found in the related publication. The current recorded during cyclic voltammetry was collected in several Excel files. Each Excel file contains a sheet with meta data. The following sheets contains the CV data associated to the experiments listed on the meta data sheet. The reactor was cleaned and the electrode was thoroughly polished between some experiments, however this was not the case for all experiments. In some cases, the solution was only stirred between experiments. This is documented in the meta data as clusters. Different clusters are nominated using letters (A, B, C, etc) and the order of execution using numbers (1, 2, 3, etc). Cyclic voltammetry was performed by cycling the current between E start and E end, starting at E start. Potentials are noted as V vs Ag/AgCl reference electrode.  

********This dataset contains the following files:********
Data_buffer.xlsx – This file contains the CV data (current in A and potential in V vs Ag/AgCl) of 1 M bicarbonate buffer. The metadata contains: Experiment number, date, E start (V), E end (V), scan rate (mV/s), rotation rate (rpm), CV cycles performed, cluster data order of magnitude of logged current (mA or µA), and notes.
Data_(thio)sulphate_sulphite_sulphur.xlsx - This file contains the CV data (current in A and potential in V vs Ag/AgCl) of thiosulphate, sulphate, and sulphite solutions solutions and biosulphur suspensions. The metadata contains: Experiment number, date, sulphur species investigated, sulphur species concentration, E start (V), E end (V), scan rate (mV/s), rotation rate (rpm), CV cycles performed, cluster, order of magnitude of logged current (mA or µA), and notes. 
Data_sulphide.xlsx - This file contains the CV data (current in A and potential in V vs Ag/AgCl) of sulphide solutions. The metadata contains: Experiment number, date, sulphide concentration (mM), E start (V), E end (V), scan rate (mV/s), rotation rate (rpm), CV cycles performed, cluster, order of magnitude of logged current (mA or µA), and notes. 
Data_polysulphide.xlsx - This file contains the CV data (current in A and potential in V vs Ag/AgCl) of polysulphide solutions. The metadata contains: Experiment number, date, sulphide concentration (mM), E start (V), E end (V), scan rate (mV/s), rotation rate (rpm), CV cycles performed, cluster, order of magnitude of logged current (mA or µA), and notes.
Data_SOB.xlsx - This file contains the CV data (current in A and potential in V vs Ag/AgCl) of SOB suspensions, either with or without incubation with sulphide. The metadata contains: Experiment number, date, SOB batch, SOB concentration (mgN/L), sulphide added (mM), sulphide concentration after incubation (mM). E start (V), E end (V), scan rate (mV/s), rotation rate (rpm), CV cycles performed, cluster, order of magnitude of logged current (mA or µA), and notes. Different SOB batches were used. Either SOB were harvested from reactor effluent originating from a pilot biodesulphurisation plant at Wageningen University (Pilot) or a lab scale biodesulphurisation setup at Wetsus, Leeuwarden (Wetsus). Different batches of SOB are denoted by their source (Pilot or Wetsus) and month of harvest. 
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This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.
