README file for the dataset belonging to the study Effects of beta-Glucans and resistant starch on fermentation of recalcitrant fibers in growing pigs. 

Study details:
Effects of the presence of beta-glucans and resistant starch in diets on nutrient and fiber degradability of rapeseed meal [RSM] (Brassica napus) and corn Distillers Dried Grain with Solubles (DDGS) were tested in a 2  3 factorial arrangement. Two basal diets, containing either 500 g/kg RSM or DDGS and ~400 g/kg corn starch were formulated to meet or exceed nutrient requirements of growing pigs. Corn starch was partly replaced with the beta-glucan-extract (beta-GLUC; 60 g/kg w/w) or completely replaced with retrograded tapioca (RG; 400 g/kg w/w), resulting in 6 dietary treatments. A total of 10 crossbred barrows (initial body weight, 28  1.4 kg (SD); Duroc  Large White/Landrace; Hypor, Inc., Regina, SK, Canada) were fed the 6 experimental diets. In total, 46 observations were obtained in 10 pigs over 7 periods in an incomplete 10  7 Youden square. Each of 7 sequential 14 days experimental periods consisted of a 9 days adaption to the diets followed by 2 days collection of feces, 2 days collection of ileal digesta for digestibility measurements, and 1 day collection of ileal digesta for the measurement of retention time. Pigs were weighed weekly during the experiment after consuming their morning meal. Feces were collected from 08.00 to 17.00 h using bags attached to rings glued around the anus. Bags were collected within 1 h after defecation and immediately frozen (-20 C). Ileal digesta for digestibility measurements were collected from 08.00 to 17.00 h into plastic bags (10 cm in length and 4 cm in diameter). The bags were removed when filled approximately 70 % with digesta, or after a maximum of 1 h, and immediately frozen (-20 C). For retention time measurements, on day 14 of each experimental collection, 3.4 g Cr as Cr2O3 (solid phase marker) and 3.4 g Co as Co (II)-EDTA (soluble phase marker) were mixed into the morning meal. Digesta were collected at 45, 90, 180, 270, 360, 540, and 720 min after feed consumption.
For more details about the experimental design see: de Vries et al. 2016 (https://doi.org/10.1371/journal.pone.0167624)

The dataset consists of the following files:
Treatments.sas7bdat
Dig.sas7bdat
Transit.sas7bdat
Vis.sas7bdat

Treatments.sas7bdat
Data describing the sample layout. Each individual observation is characterized by an observation ID (Obs), followed by a record of the pig ID number of the pig in which the measurement was done (Pig) and its pen number (Pen), the consecutive period of the Youden square design in which the measurement was done (Period), the diet code (Diet), fibrous ingredient in the diet (Source), where RSM is rapeseed meal and 
DDGS is Distillers Dried Grain with Solubles, and fiber source added to the diet (Fiber), where control is native maize starch, gluc is beta-glucan-extract, and RS is resistant starch (retrograded tapioca).

Dig.sas7bdat
Data describing ileal and total tract digestibility (%), where IDIG_DM is ileal digestibility of dry matter, IDIG_fat is ileal digestibility of crude fat, IDIG_CP is ileal digestibility of crude protein, IDIG_Starch is ileal digestibility of starch, IDIG_bgluc is ileal digestibility of beta-glucans, IDIG_NSP is ileal digestibility of non-starch polysaccharides, IDIG_NGP is ileal digestibility of non-glucosyl polysaccharides, IDIG_resglc is ileal digestibility of residual glucosyl polysaccharides, IDIG_Ara is ileal digestibility of arabinosyl residues, IDIG_Xyl is ileal digestibility of xylosyl residues, IDIG_Glc is ileal digestibility of glucosyl residues, IDIG_UA is ileal digestibility of uronyl residues, FEDIG_DM is total tract digestibility of dry matter, FEDIG_fat is total tract digestibility of crude fat, FEDIG_CP is total tract digestibility of crude protein, FEDIG_Starch is total tract digestibility of starch, FEDIG_bgluc is total tract digestibility of ?-glucans, FEDIG_NSP is total tract digestibility of non-starch polysaccharides, FEDIG_NGP is total tract digestibility of non-glucosyl polysaccharides, FEDIG_resglc is total tract digestibility of residual glucosyl polysaccharides, FEDIG_Ara is total tract digestibility of arabinosyl residues, FEDIG_Xyl is total tract digestibility of xylosyl residues, FEDIG_Glc is total tract digestibility of glucosyl residues, and FEDIG_UA is total tract digestibility of uronyl residues.

Transit.sas7bdat
Data describing ash (g/100g), dry matter (DM, g/100g), chromium (Cr, mg/kg), and cobalt (Co, mg/kg) contents.  

Vis.sas7bdat
Data describing viscosity measured in ileal digesta supernatant, where Vis_1 is the average viscosity (mPa*s) measured at 1.29, 12.9, and 129 s-1 at 20C, Vis_2 is the viscosity (mPa*s) measured at 129 s-1 at 20C, Vis_3 is the average viscosity measured at 1.29, 12.9, and 129 s-1 at 20C after heating the supernatant (80C, 10 min), Vis_4 is the viscosity (mPa*s) measured at 129 s-1 at 20C after heating the supernatant (80C, 10 min), logvis is log(Vis_4), logvish is log(Vis_4) and invvish the inverse of Vis_4.  
