1. Introductory information

Title
Data underlying the publication: Neuroprotective Effects of Everolimus via Regulation of Autophagy-Related Proteins in a Cerebral Ischemia-Reperfusion Rat Model

Description
The data was collected from MCAO rats to evaluate the neuroprotection of everolimus. In excel file, the Longa’s neurological scale was applied to evaluate neurobehavioral deficit in ischemia/reperfusion-induced rats, and the ischemic infarct area of each section was calculated as infarct size. Besides, immunochemical staining of MCAO rats for mTOR and Beclin-1 expression was uploaded to observe the influence of everolimus on mTOR and Beclin-1. Besides, the data also included WB images of pPI3K, PI3K, pAkt, Akt, LC3-I, LC3-II. This study explored the effect of everolimus on the expression of PI3K, LC3, and Akt proteins and further clarified the regulatory mechanism of nerve cell autophagy against neural lesions.

File naming convention
The experiment method-target protein or mRNA gene.

Format of the file
*.xlsx, *.tif, *.rar, *.png

The relationship between the files
Both files with the titles of wb-10 and wb-20 were the scanning image of PI3K in Western blot assay. Other files are separative file for each experiment and target protein. 

2. Methodological information

The assessment of infarct size and neurobehavioral deficit of ischemic rats was performed at 24 hours after surgery by TTC staining and Longa’s neurological test. Neurobehavioral deficit of MCAO rats was scored as follows: 1) walking normally without obvious impairment (0 score); 2) the contralateral forelimb could not stretch completely (1 score); 3) walking in contralateral circling (2 score); 4) tumbling while walking (3 score); 5) cannot walk with low consciousness (4 score). For the measurement of infarct size, the brain was cut into five slices, which were incubated in 0.5% TTC buffered in PBS (pH 7.4) at 37°C for 15 min before scanning. The formula for hemispheric infarct: percentage (%)= (contralateral hemispheric area - ipsilateral hemispheric area)/contralateral hemispheric area × 100%. The infarct size was calculated as the mean value of all five consecutive sections in two sides.

Others are raw data as shown in the manuscript.

3. Data specific information

Full names and definitions of column
Column headings in excel are well defined.

Units of measurement
Units of measurement for infarct size is percentage (%), for neurobehavioral score is score (0-4).

Definitions for codes or symbols used to record missing data
The blank or "—" is defined as missing data.

Specialized formats or abbreviations used
Both files with the titles of wb-10 and wb-20 were the scanning image of PI3K in Western blot assay.

4. Sharing and Access information
There is no restrictions placed on the data.

