Title of the dataset:
Data underlying the publication: Variation of viscoelastic properties of extracellular polymeric substances and their relation to anaerobic granule’s mechanical strength in full-scale treatment plants


Creators:
Chang Gao. Department of Environmental Technology, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.


Contributors:
Dainis Sudmalis. Department of Environmental Technology, Wageningen University and Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.


Related publications:
Variation of viscoelastic properties of extracellular polymeric substances and their relation to anaerobic granule’s mechanical strength in full-scale treatment plants
https://doi.org/10.1016/j.biortech.2024.131233

Description:
In this contribution, we characterized the viscoelastic properties of EPS from granules of 9 full-scale industrial anaerobic reactors; 
and quantitatively investigated whether these properties correlate with granules’ resistance to compression (Egranule) and shear strength (Sgranule); 
Additionally, we also characterized the EPS amount and composition from different granules.

Keywords:
Anaerobic granules; Extracellular polymeric substances; Mechanical strength; Rheology

Spatial coverage:
the Netherlands

Files:

1) Data in Sheet 1 - Reactors: is corresponding to Table 1 in the manuscript.

2) Data in Sheet 2 - Granule TSVS: is for the calculation of granule strength and EPS composition.

3) Data in Sheet 3 - EPS amount: is for the calculation of EPS content in Fig. 1 (right axis).

4) Data in Sheet 4 - EPS composition: is for the calculation of PN/PS ratio in EPS in Fig. 1 (left axis).

5) Data in Sheet 5- Shear strength_granule is for the calculation of Sgranule in Fig. 2-a. The raw data of cumulative fines production (XF) in each time interval is shown in this sheet. Sgranule is calculated as Equations [1]-[3] in the manuscript.

6) Data in Sheet 6- Compression strength_raw is the raw data to calculate Egranule in Fig. 2-b. The force (F(x)) and distance (x) measured by a texture analyzer during compression are shown in this sheet. 

7) Data in Sheet 7- Compression strength_process shows the granule radius (R) measured by a macroscopy and the calculated Egranule as the Equation [4] in the manuscript. The results are plotted in Fig. 2-b.
 
The modelled F (x) with the calculated Egranule is compared with the measured F(x) in Fig. S1. The correlation between Egranule and Sgranule is plotted in Fig. 3.

8) Data in Sheet 8 - EPS rheology summarizes the full dataset of moduli changes with varied time/ frequency/shear strain during Time Sweep, Frequency Sweep, and Amplitude Sweep. The results are plotted in the 1st ~ 3rd rows of the figures in this sheet.
 Additionally, the modelling results of softening rates during the amplitude sweep are illustrated in this Excel sheet (the 4th Row of the figures). 
 The operation parameters are shown in Table 2 in the manuscript. The moduli changes during the time sweep and frequency sweep are shown in Fig. 4 (a) and Fig. 4 (b) in the manuscript as examples. The moduli changes during the amplitude sweep are shown in Fig. 5 (a) and Fig. 5 (b) in the manuscript as examples. 
 The data under Frequency Sweep in the sheet is corresponding to Fig. S2 and Table S1 in the manuscript. The data under Amplitude Sweep is corresponding to Fig. S3 and Table 3 in the manuscript. The correlations between EPS viscoelastic properties/content and granule strength are compared in Fig. 6 in the manuscript.

9) Data in Sheet 8 – Protein secondary structure summarizes the relative abundancy of the α-helices and β-sheets structure in the proteins. The data is plotted in the Fig. S4 in the manuscript.

Explanation of abbreviations and variables

The abbreviations and variables are explained in the dataset.

Licence:

Variation of viscoelastic properties of extracellular polymeric substances and their relation to anaerobic granule’s mechanical strength in full-scale treatment plants © 2024 by Chang Gao is licensed under CC BY 4.0. 
