Data underlying the publication:  Heterogeneity and genomic plasticity of clinical isolates of 
Acinetobacter baumannii and Acinetobacter nosocomialis from India
Authors: Manasa Tantry, Chaitanya Tellapragada, Tushar Shaw, Shwethapriya Rao, Chiranjay 
Mukhopadhyay, Vandana Kalwaje Eshwara
Corresponding Author: Vandana Kalwaje Eshwara
Contact Information: manasa.tantry@learner.manipal.edu
Department of Microbiology, Kasturba Medical College, Manipal, Manipal Academy of Higher 
Education, Manipal, India  576104
*** General Introduction ***
This dataset contains data generated from experiments performed at Department of Microbiology, 
Kasturba Medical College, Manipal as part of Manasa Tantrys PhD Thesis project (December 2023). It 
is being made public to act as supplementary data for publication. This research project was made 
possible by a grant from the Dr. TMA PAI Endowment Award to Dr. Vandana Kalwaje Eshwara.
***Objective***
The objective of this research was to elucidate the pathogenic characteristics and to evaluate the in 
vitro efficacy of antibiotic combinations in demonstrating synergy.
***Study design***
Employing a mixed-methods approach, the data was collected through laboratory-based 
experimental procedures, including growth curve assays to monitor bacterial proliferation, biofilm 
formation assays to assess the capacity to form biofilms, checkerboard assays for determining the 
synergy between different antimicrobials, and time-kill kinetics experiments to evaluate the 
effectiveness of antibiotics over time.
***Methods***
Growth kinetics assay was performed in 96 welled microtitre plate with Optical density (OD) read at 
600nm every 30 minutes for 48 hours. Biofilm formation was evaluated by the crystal violet assay in 
quadruplicates and the optical density of the solubilized biofilm were measured at 600 nm. 
Checkerboard assay was performed to assess the fractional inhibitory concentration (FIC) of 
antibiotic combinations and were interpreted as synergistic/indifferent/antagonistic and time-kill 
assays (TKA) were conducted to assess the synergistic/bactericidal activity of the antibiotic 
combinations. The killing curves were generated by plotting the viable count (log10 CFU/mL) at 
regular intervals over 24h.
***Description of the data in this data set***
The dataset contains quantitative measurements facilitating a comprehensive analysis of the 
pathogenic behaviour of Acinetobacter species and their interactions with antimicrobial agents.
1.	Growth curve assay  OD 600 values and growth rate
2.	Biofilm assay - OD 600 values and interpretation
3.	Checkerboard assay  FIC values
4.	Time-kill assay  Viable counts (log 10 Colony forming units/mL) over 24 hours
