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This is the dataset of the study, Prominent Obesity-induced Systemic Inflammation Exacerbates Lupus Activity and Organ Damages in Fc Gamma Receptor IIb Deficient Lupus Mice, Impacts of Saturated Fatty Acid and Gut Barrier Defect. We compared the systemic inflammation in a FcgRIIb deficient (FcgRIIb-/-) lupus mouse model, between with and without HFD-induced obesity. We also conducted the in vitro macrophage experiments to test the impact of endotoxin and saturated fatty acid on lupus condition. 

File 1 
Characteristics of wild type (WT) or Fc gamma receptor IIb deficient (FcgRIIb-/-) macrophages after activation by control media (DMEM), palmitic acid saturated fatty acid (PA), lipopolysaccharide (LPS), and PA with LPS (PA+LPS)

The variables include;
Visualized lipid containing cells; in percents of total cells per field
Supernatant TNF-?; in pg/mL
Supernatant IL-6; in x10^2 pg/mL
Supernatant IL-10; in x10^2 pg/mL
iNOS expression; in times relative to beta-actin
IL-1beta expression; in times relative to beta-actin
Arginase-1 expression; in times relative to beta-actin
TGF-beta expression; in times relative to beta-actin
Dihydroethidium (DHE); in times relative to DMEM control

File 2
Characteristics of wild type (WT) or Fc gamma receptor IIb deficient (FcgRIIb-/-) mice with regular diet (RD) or high-fat diet (HFD)

The variables include;
Body weight; in gram
Serum Creatinine; in mg/dL
Serum FITC-dextran assay; in x10^2 ng/mL
Endotoxemia; in EU/mL
Serum IL-6; in pg/mL
Total cholesterol; in pg/mL
High-density lipoprotein; in mg/dL
Mesenteric fat; in x10^2 pg/mL; in gram
Perigonadal fat; in grams
Perirenal fat; in grams
Subcutaneous fat thickness; in micrometer
Alanine transaminase; in U/L
Liver weight; in grams
Liver injury score; in score
Hepatic fibrosis; in percents area per high power field
Hepatocyte apoptosis
Liver TNF-a; in pg/g
Liver IL-6; in pg/g
Liver IL-10; in pg/g
Kidney injury score; in score
Renal fibrosis; in percents area per high power field
Kidney TNF-a; in pg/g
Kidney IL-6; in pg/g
Kidney IL-10; in pg/g
Spleen activated caspase 3; in cells per high power field
Aorta thickness; in micrometer

File 3
Characteristics of cell energy alteration in extracellular flux analysis (without reagents for energy manipulation) in wild type (WT) or Fc gamma receptor IIb deficient (FcgRIIb-/-) macrophages preconditioned by palmitic acid saturated fatty acid (PA) or control media (DMEM) followed by lipopolysaccharide (LPS) stimulation

The variables include;
OCR; oxygen consumption rate in pmol/min/mg
ECAR; extracellular acidification rate (mpH/min/mg)
AUC of OCR; area under curve of oxygen consumption rat (standardized by pre-injection of LPS)
AUC of ECAR; area under curve of extracellular acidification rate (standardized by pre-injection of LPS)
Total ATP; in nM
Mitochondrial mass; in MFI
Mitochondrial membrane potential; in MFI
