***Data underlying the PhD thesis: Ecology, metabolism and energetics of "Ca. Accumulibacter"***
Authors: T. Paez-Watson, M.C.M. van Loosdrecht, S. A. Wahl
Department of Biotechnology, Faculty of Applied Sciences, Delft University of Technology


*** General description ***
Data that has been used for the publication of the PhD thesis.

All software and simluation data has been previously published with each chapter 
in GitHub. This dataset includes non-available experimental data from the reactor 
analysis of each publication.


References from publication:

- https://doi.org/10.1021/acsestwater.4c00247?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-as
- Metabolic implications for dual substrate growth in “Candidatus Accumulibacter”. 
Article under review in Environmental Science and Technology


This work was supported by SIAM Gravitation Grant 024.002.00, the Netherlands 
Organization for Scientific Research (NWO).



*** Data collection and file description ***

1) ------ Sweet Secrets qFISH ------

Microscopic images from the 2 bioreactor enrichments of "Candidatus Accumulibacter"
probed with the FISH oligonucleotides PAOmix, GAOmix and EUBacteria. Together with 
the FISH images, the python code that was developed and used to quantify the cells
of different colors for qFISH. 
More details regarding the experiments and the microscopic images at:
are available at https://doi.org/10.1021/acsestwater.4c00247?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-as


2) ------ Sweet Secrets Reactor Operation ------

The operational data of the long term bioreactot enrichments run for the publication.
Both bioreactor enrichments were operated under the exact same parameters except for 
the rotational speed at 400 and 800 rpms respectively. 

Available files:
- Time_samples.xsls  Cycle analyses on the reactors at steady state
- TSS.VSS measurements on the biomass concentration of the reactors over time
- HPLC results.xsls  HPLC results and phosphate measurements of cycle analysis.


3) ------ Experiments Mix Substrates ------

The operational data of the long term bioreactor enrichment run for the publication.
Bioreactor was enriched under acetate as the only carbon source, but was tested in 
batch mode for it's uptake capacities of acetate, aspartate and a mix. Files contain
data regarding the batch tests with mixed substrate.

Available files:
- FISH images of the community
- Cycle studies performed in January and February during problems of the reactor operation.
- Batch tests 2023. Batch tests of the mixed substrates. Results indicate PHAs, glycogen, polyphosphate and acetate/aspartate consumption over time.