********Data underlying the research of Efficient Hydrothermal Pretreatment of Mixed PHA/PLA and Consequent Continuous Anaerobic Fermentation into VFA********
Authors: Yong Jin 1, Ralf Beckmans 1, Kasper D. de Leeuw 1, and David P. B. T. B. Strik 1
1	Environmental Technology, Wageningen University & Research, Axis-Z, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; yong.jin@wur.nl 


Corresponding author: David P. B. T. B. Strik
david.strik@wur.nl

Wageningen University & Research - Environmental Technology
Bornse Weilanden 9
6708 WG Wageningen
the Netherlands


********General Introduction********
This dataset contains data collected during PHA and PLA pretreatment and hydrolysates fermentation experiments at Wageningen University & University as part of Yong Jin's PhD thesis project (November 2021): 
Doi: 
This information is being made public as supplementary data for the publications and PhD thesis of Yong Jin, and other researchers are encouraged to utilize this data in their work. 

The data in this dataset was collected in the Environmental Technology laboratory of Wageningen University & Research between November 2021 and December 2025. 
This research project was made possible by a grant from the China Scholarship Council (CSC). 

********Purpose of the test campaign********
These experiments aimed to investigate the feasibility of valorizing biobased biodegradable plastics into carboxylates via tandem hydrothermal processing and open-culture fermentation. 
The data shown in this file can validate the results. 


********This dataset contains the following files:********
1_Pretreatment.xlsx contains four sheets that summarize key data related to the hydrothermal pretreatment of PHA/PLA bioplastics:  
	The first sheet, "Constants": The fundamental chemical constants used in calculations, including molecular weight, carbon atoms, soluble COD, and degree of reduction (electrons/carbons) for both substrates and hydrolysis products; 
	The second sheet, "Compositions analysis": The measured concentrations of PHA and various supplemental compounds, as well as the composition and relative percentages of various hydrolysates; 
	The third sheet, "Temperature": The PHA dissolution yields obtained under different combinations of temperature and reaction time; 
	The fourth sheet, "Hydrolysates in fermentation": The concentrations of hydrolysates–specifically 3-hydroxybutyrate (3-HB), crotonate, and lactate–quantified during different fermentation phases. It also includes the calculated hydrolysis yields based on the initial PHA/PLA pellet content. 

		
2_Dataset for the CSTR.xlsx contains thirteen sheets that document the experimental design, raw data, and analytical calculations from the continuous stirred tank reactor (CSTR) experiments for the fermentation of PHA and PLA hydrolysates: 
	The first sheet, "Constants": the fundamentals of chemicals used for calculation, including molecular weight, carbon atoms, soluble COD values, and degree of reduction (electrons/carbons) for both substrates and fermentation products; 
	The second sheet, "Experimental plan": the experimental design parameters for CSTR operation, such as pH, temperature, substrate compositions, and methane inhibitor addition/removal; 
	The third sheet, "Rawdata": lab journal entries and routine operational check-ups during the CSTR run; 
	The fourth sheet, "Influent Concentration": the measured concentrations of influent substrates, including 3-hydroxubutyrate, crotonate, lactate, yeast extract, and ethanol; 
	The fifth sheet, "Effluent Concentration": the concentrations of residual substrates (3-hydroxybutyrate, crotonate, ethanol, lactate) and produced compounds such as acetate, propionate, i-/n-butyrate, n-valerate, n-caproate, n-butanol; 
	The sixth sheet, "Average concentrations": the calculated net concentrations of substrates and products at each steady-state fermentation phase; 
	The seventh sheet, "HRT": the hydraulic retention time calculations and base consumption (KOH) during fermentation; 
	The eighth sheet, "KOH needs for medium": the required amount of KOH for medium preparation before fermentation; 
	The ninth sheet, "Gas": the composition of headspace gases in the bioreactor, including H2, O2, N2, CO2, and CH4; 
	The tenth sheet, "Productivities": the calculated productivities (mmol C/day) for both substrate inflow and product outflow; 
	The eleventh sheet, "Balances": the carbon and electron balance calculations throughout the fermentation process; 
	The twelfth sheet, "Net consum-produc": the net consumption and production of key compounds at each steady-state phase; 
	The thirteenth sheet, "SCOD": the measurements of filtrated soluble COD in both influent and effluent samples. 


3_NGS CSTR 16S.xlsx contains next-generation sequencing (NGS) data of 16S rRNA gene amplicons, providing insights into microbial community dynamics during the CSTR fermentation process: 
	The first sheet, "Inoculum": microbial community analysis of the inoculum, presented as relative abundance from the kingdom to the species levels; 
	The second sheet, "Phase I": microbial community analysis of the fermentation broth at the end of Phase I (relative abundance); 
	The third sheet, "Phase III": microbial community analysis of the fermentation broth at the end of Phase III (relative abundance); 
	The fourth sheet, "Phase IV": microbial community analysis of the fermentation broth at the end of Phase IV (relative abundance); 
	The fifth sheet, "Phase V": microbial community analysis of the fermentation broth at the end of Phase V (relative abundance); 
	The sixth sheet, "Phase VI": microbial community analysis of the fermentation broth at the end of Phase VI (relative abundance); 
	The seventh sheet, "Phase VII": microbial community analysis of the fermentation broth at the end of Phase VII (relative abundance); 
	The eighth sheet, "16S rRNA mappingstats": the percentage of sequenced reads successfully classified across different taxonomic levels for each fermentation phase. 


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This dataset is published under the CC BY (Attribution) license.
This license allows users to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.

