********Microbial recycling of bioplastics via mixed culture fermentation of hydrolyzed polyhydroxyalkanoates into carboxylates********
Authors: Yong Jin 1, Kasper D. de Leeuw 1,2, and David P. B. T. B. Strik 1
1	Environmental Technology, Wageningen University & Research, Axis-Z, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; yong.jin@wur.nl 
2	Chain Craft B.V., Basisweg 68, 1043 AP Amsterdam, the Netherlands; kasper.deleeuw@wur.nl

Corresponding author: David P. B. T. B. Strik
david.strik@wur.nl

Wageningen University & Research - Environmental Technology
Bornse Weilanden 9
6708 WG Wageningen
the Netherlands


********General Introduction********
This dataset contains data collected during PHBV fermentation at Wageningen University & University, as part of Yong Jin's PhD thesis project (November 2021): 
doi: 10.4121/21904716
It is being made public both to act as supplementary data for publications and the PhD thesis of Yong Jin and in order for other researchers to use this data in their own work. 

The data in this dataset was collected in the Environmental Technology laboratory ofr the Wageningen University & Research, between November 2021 and December 2025. 
This research project was made possible by a grant from the China Scholarship Council (CSC). 

********Purpose of the test campaign********
The purpose of these experiments was to identify the possibility of PHA biodegradation to VFAs after hydrolysis. 
The data as shown in this file can validate the efficiency of thermochemical pretreatment and microbial fermentation in bioplastic recycling. 


********This dataset contains the following files:********
PHA to VFA.xlsx - the first sheet "pH change": pH variation during fermentation, "R" means fermentation groups, and "C" means control groups; 
                  the second sheet "VFAs concentration": the concentration during fermentation (in g/L); 
                  the third sheet "convert VFAs to SCOD": the SCOD variation during fermentation (in gCOD/L), that means converting concentration of VFAs into SCOD (soluble chemical oxygen demand); 
                  the fourth sheet "chemical pretreatment": the SCOD variation after three different pretreatments (in gCOD/L)-acidific pretreatment (1 M HCl), basic pretreatment (1 M KOH), and control group (demi water); 
                  the fifth sheet "alkaline pretreatment": the COD change from theoretical COD of PHBV to soluble COD of dissolved PHBV (gCOD/L), the PHBV means polyhydroxybutyrate-co-valerate); 
                  the sixth sheet "sampling SCOD": the SCOD variation during fermentation with different dilutors (in gCOD/L); 
                  the seventh sheet "Gas pressure": the variation of gas pressure for every sampling (in bar); 
                  the eighth sheet "Gas composition": the comparison of gas components between the start and the end of fermentation (in %), maily focus on O2, N2, CO2, H2; 
                  the ninth sheet "Temperature": the Temperature control from hydrolysis to fermentation (in ℃); 
                  the tenth sheet "Raw data of VFAs": the data collected from gas chromotography (in g/L);
                  the eleventh sheet "Raw data of crotonate and 3-HB": the raw data about the comparison of fermentation group and control group in the retention time and area for crotonate and 3-hydroxybutyrate (3-HB).


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This dataset is published under the CC BY (Attribution) license.
This license allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator.

