README for FigureS3A_data.csv

*** This file contains the raw data obtained on DNA-HMfB complexes using magnetic tweezers as represented in Figure S3A of

Article: Mechanical and structural properties of archaeal hypernucleosomes
Authors: Henneman, Brouwer, Erkelens, Kuijntjes, van Emmerik, van der Valk, Timmer, Kirolos, van Ingen, van Noort, Dame
Journal: Nucleic Acids Research
DOI: 10.1093/nar/gkaa1196
Corresponding authors: rtdame@chem.leidenuniv.nl and noort@physics.leidenuniv.nl

Legend Figure S3) Release curves of torsionally constrained HMfB-DNA complexes. A) Force spectroscopy on negatively twisted HMfB-DNA complexes. The release curves suggest that overtwisted DNA has higher affinity to wrap DNA onto the HMfB dimers compared to relaxed DNA, shown by the reduced extension of the release curve. As a reference, the stretch curve for 0 twist was added in bright blue. Upon negative twist, the hysteresis reduced. B) Force spectroscopy experiments on positively twisted HMfB-DNA complexes. At forces between 10 and 40 pN the extension was reduced with increasing twist, which was also observed in the pulling curve.  

*** The data were obtained using Magnetic Tweezers as described in the associated article.

*** Columns show F (Force in pN) and z (Distance in micrometer) of DNA-HMfB complexes. Each column label additionally indicates the amount of negative twist induced.
