README for Figure5B_data.csv

*** This file contains the raw data obtained on DNA-HMfB complexes using magnetic tweezers as represented in Figure 5B of

Article: Mechanical and structural properties of archaeal hypernucleosomes
Authors: Henneman, Brouwer, Erkelens, Kuijntjes, van Emmerik, van der Valk, Timmer, Kirolos, van Ingen, van Noort, Dame
Journal: Nucleic Acids Research
DOI: 10.1093/nar/gkaa1196
Corresponding authors: rtdame@chem.leidenuniv.nl and noort@physics.leidenuniv.nl

Legend Figure 5) Stretching a torsionally constrained HMfB-DNA complex reveals left-handedness of the hypernucleosome, and shows increased affinity of HMfB dimers for positively twisted DNA. A) Negative twist as a result of 5-25 rotations increases the unstacking force of the hypernucleosome. In addition, excess negative twist causes the HMfB-DNA complex to buckle, resulting in a dramatic decrease of its extension. Both observations point to the left-handedness of the hypernucleosome. The unwrapping transition appears largely unaffected, resulting in a continuous decondensation of the tether around 10 pN. B) Positive twist as a result of 5-25 rotations recovers the unstacking plateau. Strikingly, positive twist increases the rupture force of the unwrapping transition of HMfB dimers. An increasing force is necessary to reverse the bending effect of HMfB dimers on DNA. At very low forces the beads appear to stick to the cover slip for certain curves (-5, -20, and +20 rotations, especially).

*** The data were obtained using Magnetic Tweezers as described in the associated article.

*** Columns show F (Force in pN) and z (Distance in micrometer) of DNA-HMfB complexes. Each column label additionally indicates the amount of positive twist induced.
