README for Figure3A_data.csv

*** This file contains the raw data obtained on DNA and DNA-HMfB complexes using magnetic tweezers as represented in Figure 3A of

Article: Mechanical and structural properties of archaeal hypernucleosomes
Authors: Henneman, Brouwer, Erkelens, Kuijntjes, van Emmerik, van der Valk, Timmer, Kirolos, van Ingen, van Noort, Dame
Journal: Nucleic Acids Research
DOI: 10.1093/nar/gkaa1196
Corresponding authors: rtdame@chem.leidenuniv.nl and noort@physics.leidenuniv.nl

Legend Figure 3) Force spectroscopy experiments on the hypernucleosome reveal stronger stacking in HMfB tethers than in HMfA tethers. A) Force spectroscopy on a HMfB-DNA complex (blue dots) at 100 nM of HMfB reveals three levels of compaction. A bare DNA molecule is shown in yellow dots. We fitted different parts of the curve to a freely-jointed chain (FJC) (I), kinked worm-like chain (WLC) (II), and WLC (III). Fixed  parameters per stacked dimer in regime I: force independent length = 0.5 nm, FJC contour length = 4 nm, length of wrapped DNA = 30 bp.  Fixed parameters for DNA in regime III: contour length = 3646 bp, DNA persistence length = 50 nm, DNA stretch modulus = 900 pN. The release curves overlapped with the stretch curves, which indicates that HMfB-DNA the stretch-release cycle was in equilibrium. B) Comparison between HMfA hypernucleosomes (red dots) and HMfB hypernucleosomes (blue dots). Each curve shows a pulling trace in color and a refolding curve in grey, typically largely obscured by overlap with the pulling curve.  C) Structural models of three states of the HMfB-DNA complex corresponding to the model fit in A, illustrating a 7-fold compaction of the hypernucleosome compared to bare DNA. D-G ) Histogram of fit parameters to equation 10 for HMfA and HMfB complexes.

*** The data were obtained using Magnetic Tweezers as described in the associated article.

*** Columns show F (Force in pN) and z (Distance in micrometer) of bare DNA and DNA-HMfB complexes
