README for Figure2_HMfA.xlsx

*** This file contains the raw data obtained on DNA and DNA-HMfA complexes using tethered particle motion represented in Figure 2 of

Article: Mechanical and structural properties of archaeal hypernucleosomes
Authors: Henneman, Brouwer, Erkelens, Kuijntjes, van Emmerik, van der Valk, Timmer, Kirolos, van Ingen, van Noort, Dame
Journal: Nucleic Acids Research
DOI: 10.1093/nar/gkaa1196
Corresponding authors: rtdame@chem.leidenuniv.nl and noort@physics.leidenuniv.nl

Legend Figure 2: Methanothermus fervidus histone proteins HMfA and HMfB cooperatively compact DNA in Tethered Particle Motion experiments. Root mean square displacement (RMS) excursion of the bead for HMfA and HMfB on a DNA substrate is shown as a function of protein concentration. Dashed lines are to guide the eye. Error bars indicating the standard error of the data points (N≥100) are small and mostly hidden behind the data points. In grey, saturation levels of the bacterial NAP HU and archaeal NAPs Sso10a1 and Cren7 – which are known to organize and compact DNA – are indicated, as reported by Driessen et al. 2014, Driessen et al. 2016 and Driessen et al. 2013.

*** The data were obtained using Tethered Particle Motion as described in the associated article.

*** Data obtained at each HMfA concentration is given on an individual sheet. The sheet labelled “overview” contains a summary of processed data as plotted in Figure 2.
Each individual sheet reports on an individual tethered DNA molecule or protein-DNA complex
Column A: data_overview = RMS in nm
Column B: anisotropic = anisotropic ratio (movement in x-direction/movement in y-direction)
Column C: stdev = standard deviation
Column D: FoV = field of view (data with the same FoV were collected in one image)
Column E: bead = beads in each FoV are represented by a unique number
Column I: RMS = average RMS values as obtained by fitting a normal distribution to the RMS distribution of replicates (Set 1 - X)

The values given in column B and C are tether quality criteria and used for data filtering as described in the associated article.
Data from replicates is shown using different colours of shading
Fits of normal distributions to the data of replicates are shown for reference.


 
