README for Figure 2A.csv 
*** This file contains the raw data obtained on DNA, DNA-Rok complexes using bridging assay experiments represented in Figure 2A of 
Article: The B. subtilis Rok protein is an atypical H-NS-like protein irresponsive to physico-chemical cues
Authors: Erkelens, Qin, van Erp, Miguel-Arribas, Abia, Keek, Markus, Cajili, Schwab, Meijer and Dame
Journal:  
DOI:
Corresponding author: rtdame@chem.leidenuniv.nl; wmeijer@cbm.csic.es


Legend Figure 2:Rok exhibits DNA bridging activity, which is only mildly affected by temperature, pH and 
salt concentration A) DNA recovery (as a percentage of the input DNA) as a function of Rok concentration 
from 0 to 0.5 µM as measured using the DNA bridging assay in the presence of 50 mM KCl at 25 ºC (red) and 37ºC 
(blue), respectively. B) DNA recovery as a function of pH from 6 to 10 in the presence of 0.27 µM Rok at 25 ºC. 
Data are plotted as mean values of three independent measurements and the error bars represent the standard 
deviation. Dashed lines are lines to guide the eye. C) DNA recovery as a function of KCl concentration from 
35 to 500 mM in the presence of 0.27 µM Rok at 25 ºC. D) DNA recovery as a function of MgCl2 concentration from 
1 to 90 mM in the presence of 0.27 µM Rok at 25 ºC  

*** The data were obtained using bridging assay experiments as described in the associated article. 

***The data labeled overview represents the values plotted in the graph of figure 2A. The raw data contains the individual replicates.
Column A: Sample number
Column B: Presence of bait DNA in sample
Column C: Presence of 32P-labeled prey DNA in sample
Column D: Amount of Rok added to sample in micromolar
Column E: Counts per minutes 
Column F: DNA recovery (%)

Columns B, C and D indicate the contents of the sample. Column E indicates the radioactivity of the sample. 
Column F indicates the DNA recovery, which is calculated by subtracting the value in column E of the control 
(sample minus bait DNA) from the sample itself. This value is then divided by the values in column E of sample 1 
and multiplied by 100%.