Title of dataset
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Data underlying the publication "Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting"

Data description
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- directionality histograms (.png)
- directionality orientation maps (.tif)
- directionality analysis (.csv)
- fluorescence microscopy images (.tif)
- gene expression analysis, qPCR data (.txt)
- raw microscopy files (.lif)
- sprouting efficiency ROIs (.tif)
- sprouting efficiency analysis (.csv)

Methodological information
--------------------------
Detailed descriptions of materials and methods are available in the publication (Tiemeijer and Ristori et al., 2022, iScience).

Omitted data points
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Directionality fits with a fit correctness value < 0.2 were omitted. These data points and ones lacking due to no cells in the region of interest have been marked with a *. 

Abbreviations
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- EFcs: efficiency of controlled sprouting 
- Fc: IgG Fc fragment
- qPCR: quantitative polymerase chain reaction
- ref: reference gene
- ROI: region of interest

Gene/protein symbols
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- Dll4: delta-like 4
- GAPDH: glyceraldehyde-3-phosphate dehydrogenase
- HPRT: hypoxanthine phosphoribosyltransferase
- Jag1: jagged1

Tools & instruments
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- .lif file use: Fiji (open source, https://imagej.net/software/fiji/)
- atmospheric plasma treatment: Corona Discharge Generator, Tantec HF)
- cell counting: Fiji Cell Counter plugin
- directionality analysis: Fiji Directionality plugin
- fluorescent microscopy: DMi8 TIRF microscope (Leica Microsystems)
- microscope software platform: LAS X small 3.4.2 (Leica Microsystems)
- quantitative PCR: QuantStudio 3 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific)
- statistical analysis and graphs: GraphPad Prism 8.0.2 (GraphPad Software)