*** Stable nitrogen isotope analysis of amino acids as a new tool to clarify complex parasite-host interactions within marine food webs***

Authors: Philip Riekenberg1*+, Tijs Joling1,2+, Lonneke L. IJsseldijk3, Andreas M. Waser2,4, Marcel van der Meer1, David W. Thieltges2 

(1) Department of Marine Microbiology & Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands
(2) Department of Coastal Systems, NIOZ Royal Netherlands Institute for Sea Research, 
PO Box 59, 1790 AB Den Burg, Texel, The Netherlands
(3) Division of Pathology, Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3854 CL, Utrecht, The Netherlands
(4) Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Wadden Sea Station Sylt, Hafenstraße 43, 25992 List, Sylt, Germany

Corresponsding Author: P. Riekenberg phrieken@gmail.com

***General Introduction***
This data set contains the carbon and nitrogen stable isotope values from the parasite-host pairs collected from the Wadden and North Seas as described in the manuscript as part of Tijs Joling's MS project.

The data in this manuscript was produced in the Stable Isotope Laboratory in the Marine Microbiology and Biogeochemistry Department at NIOZ. 

***Test Equipment***
Tissue samples were then loaded into tin capsules for Bulk-SIA analysis using a Delta V Advantage Isotope ratio mass spectrometer with a Flash 2000 Organic Element Analyzer (Thermo Fisher Scientific). 
The reference materials acetanilide (Biogeochemical Laboratories at Indiana University), urea, and casein (Microanalysis Ltd., Okehampton, UK) were standards for stable isotope measurements for δ13C 
and δ15N expressed as per mil (‰) differences from the δ13C value of Vienna Peedee-Belemnite Limestone (VPDB) and the δ15N value of atmospheric N2. Sample precision was ±0.1‰ and ±0.2‰ for δ13C 
and δ15N, respectively, for bulk materials supporting this study.   

***Description of data***
lab; laboratory that analyzed the sample
lab_sample_id; lab sample ID
user_sample_id; User sample ID
sample_measurement_id; Bulk analysis of C and N 
collected_sample_mass; sample weight
instrumentation; Elemental analyzer
analysis_type; Bulk analysis of solid material
analysis_date; date and month of analysis
client_id; Who owns the sample
analytical_matrix; all tissues in this data set
primary_reference_material; Primary isotope reference material
preparation_step; sample preparations
preparation_step; sample preparations
material_type; generalized categories for sample ID of tissues
material_sample_id; Exact sample ID for tissue types
collection_source; All field samples
investigator_name; Philip Riekenberg
investigator_orcid; 0000-0002-6275-5762
investigator_email; phrieken@gmail.com
collection_locality; Wadden or North Sea
taxon_rank; ID is to species level
scientific_name; Genus species
%C; percent  carbon
%N; percent nitrogen
d13C; delta 13 C in per mille
d13C_measurement_scale; against Vienna PeeDee Belemnite
d13C_measurement_unit; per mille
d15N; delta 15N in per mille
d15N_measurement_scale; against atmospheric N2
d15N_measurement_unit; per mille