Title of the dataset:
Pelargonium geometric morphometric data

Authors:
SJ van de Kerke (Sara)

Description:
Photographs and TPS file including landmark placement for Pelargonium species included in the SPUR and PETAL dataset. Outcome of the virtual3D reconstruction analysis.

This dataset contains the following files:
photographs and raw landmarking data in .tps files and virtual3D created landmark data for Pelargonium species



Methods:
Floral shape was compared for 90 Pelargonium species growing in living collections in The Netherlands, Germany and in South Africa 
We selected flowers with corollas and flowers with stamens (used a proxy for full anthesis of the spurs, which was confirmed by eye) in full anthesis separately to limit possible ontogenetic effects on measured shape. We digitally photographed each flower using a standardised procedure in front and side view to avoid positional effects on measured shape. For each photograph, we defined a set of landmarks to provide comprehensive coverage of the specimen. We used both primary landmarks on homologous positions as well as sliding landmarks along a curve between two fixed primary landmarks. A datafile was created using tpsUtil (Rohlf, n.d.) and landmarks were placed using tpsDig v. 232 (Rohlf, 2010).
For the side view photograph, covering the spur aspect, we defined a set of 10 landmarks and 75 sliding landmarks covering the spur outline and tracking its curvature, as well as that of the shortest, longest and an average stamen. We labelled this data set SPUR. 
For the front view photograph, we followed the corolla shape landmarks as defined by Gómez et al. (2006) and placed 32 landmarks along the outline of the corolla and the opening of the nectar spur using midrib, primary and secondary veins and petal attachment as a guide. We labelled this data set PETAL. For specimens with four petals, we assumed that for the middle anterior petal the meristem is present but does not develop (Ronse De Craene, 2018). Therefore, landmarks allocated for this petal were placed but with zero length from the missing petal base. A 5 mm scale bar was included in each picture to be able to represent all landmark coordinates on the same interval scale. 

We designed a random sampling bootstrapping method based on the SPUR and PETAL datasets and we combined all resulting 8160 virtual flowers in VIRTUAL3D (see van de Kerke et al. (2019). Capturing variation in floral shape; a virtual3D based morphospace for Pelargonium, submitted) for more detail).

Licence:
CC0