Single-Molecule Localization Microscopy (SMLM) 2D Digits 123 and TOL letters datasets
1. Introductory information
The repository contains localization microscopy data (list of coordinates) of 1, 2 and
3 Digits and a 3x4 grid (\Digits subdirectory) and T, O and L Letters (\Letters subdirectory)
DNA-origmai nanostructures imaged using PAINT technique.
The files are MATLAB .mat files that can be opened using any version of
Mathworks MATLAB software.
For further questions you can contact:
Teun Huijben
Hamidreza Heydarian or and
Bernd Rieger and
Sjoerd Stallinga
2. Methodological information
All the information regarding data acquisition, collection and analysis can be
found on the following papers:
[1] Huijben T.*, Heydarian H.*, Auer A., Schueder F., Jungmann R. , Stallinga S, Rieger, B.
Detecting structural heterogeneity in single-molecule localization microscopy data,
Nature Communications (2021).
[2] Alexander Auer, Maximilian T Strauss, Sebastian Strauss, Ralf Jungmann, nanoTRON:
a Picasso module for MLP-based classification of super-resolution data, Bioinformatics,
Volume 36, Issue 11, June 2020, Pages 3620–3622, https://doi.org/10.1093/bioinformatics/btaa154
3. Data specific information
Each dataset contains a cell array of particles. When loaded in MATLAB, you can
access each individual particle using the variable particles{1,index}. Each
cell in this array is a MATLAB structure with two fields: points and sigma. The
first field, i.e. particles{1,index}.points is an Nx2 array containing N
localization data (x and y) in camera pixel unit. The second field, i.e.
particles{1,index}.sigma which is an Nx1 array contains the localization
uncertainties in SQUARED camera pixel unit. The localization uncertainties are isotropic
in lateral direction therefore sigma_x = sigma_y.
Digits dataset contains data from experiments where the different structures were imaged separately
in different experiments (Digits\Imaged_Separately) and imaged together (Digits\Imaged_Together),
e.g. the structures were mixed and imaged in a single field-of-view.
Similarly, Letters dataset contains data from experiments where T and O letter are imaged
together (Letters\TO_Imaged_Together), T, O and L are imaged separately
(Letters\TOL_Imaged_Separately) and T, O and L are imaged together (Letters\TOL_Imaged_Together).
Details about the design of the DNA origami structures and experimental imaging settings can be
found in [1] and [2], for respectively, the Letters and Digits dataset.
4. Sharing and Access information
This dataset is made available under the Attribution-NonCommercial license
CC BY-NC.