TY - DATA
T1 - Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer.
PY - 2023/02/08
AU - Xinlei Chen
UR - https://data.4tu.nl/articles/dataset/Data_underlying_the_research_of_The_mRNA_expression_of_CACYBP_in_bladder_and_kidney_cancer_/22004108/1
DO - 10.4121/22004108.v1
KW - Siah-1 interacting protein (SIP）
KW - Calcyclin Binding Protein
KW - polymerase chain reaction (PCR)
KW - nephrology
KW - urology
N2 - <p><strong>qRT-PCR </strong></p>
<p>Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit. Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii. The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells  for analysis.</p>
ER -