TY - DATA T1 - SEM image of gelatin-alginate hydrogels crosslinked by diepoxy polyethylene glycol (PEG DE 500). Data underlying the research project "Gelatin-alginate hydrogels for hard-to-heal wounds" (GAlHyd) PY - 2023/11/07 AU - Olha MAIKOVYCH AU - Pamela PASETTO UR - DO - 10.4121/e65ed658-0d8d-4890-a252-c85a7c1c2619.v2 KW - SEM KW - Gelatin KW - Aginate KW - Hydrogel KW - Morphology KW - Pore size N2 -
Scanning electron microscopy (SEM). Hydrogel samples were placed in 2 ml Eppendorf microtubes, frozen in liquid nitrogen (T = -195.75 °С)for 15 min, then transferred to a pear-shaped flask, which was connected to freeze dryer Liofilizator Alpha 1-2 LD plus (CHRICT, Munich, Germany) equipped with a two-stage oil pump RZ 2.5 (VACUUMBRAND), pressure 0.1 mbar (10 Pa), temperature -55 °С. The dried samples were gold-covered in a nitrogen atmosphere (Pump/Gold JEOL JFC-1300), photographs of the morphology were taken on a scanning microscope (JEOL, JSM 6510 LV).
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