TY - DATA
T1 - Dataset on the content of vitamin D3 and 25-hydroxyvitamin D3 in 40 pork (Breitov breed) commodities
PY - 2021/12/30
AU - Ali Jamil Othman
AU - Ludmila G. Eliseeva
AU - Fatima M Duksi
AU - Polina G
AU - Alyona Shalankina
UR - https://data.4tu.nl/articles/dataset/Dataset_on_the_content_of_vitamin_D3_and_25-hydroxyvitamin_D3_in_40_pork_Breitov_breed_commodities/17269247/1
DO - 10.4121/17269247.v1
KW - Vitamin D3
KW - 25-hydroxyvitamin D3
KW - 25OHD3
KW - HPLC
KW - Pork
KW - Food analysis
N2 - <p><i>This dataset demonstrates the content
of vitamin D</i><i><sub>3</sub></i><i> and 25-hydroxyvitamin D</i><i><sub>3</sub></i><i> (25OHD</i><i><sub>3</sub></i><i>) in boiled, grilled, and raw pork for both lean and whole cuts</i><i> including
ribs, tenderloin, shoulder, neck, belly, and center chops</i><i>. </i><i>Total fat content in raw pork cuts is
determined by the gravimetric method.</i><i></i></p>

<p><i>Quantification analysis using reverse
phase </i><i>high performance</i><i>
liquid chromatography with UV-DAD</i><i>
connected</i><i>.</i><i> </i><i>Purification
of fatty acids from proteins was performed using a c18 cartridge, resolving
vitamin D</i><i><sub>3</sub></i><i> from its
metabolites by subjecting purified samples to polar silica cartridge,
purification from sugars by a series of two amino columns. Quantification of </i><i>vitamin D<sub>3</sub> and </i><i>25-hydroxyvitamin
D</i><i><sub>3</sub></i><i>  by injecting purified extracts on C18 SPE
column.</i><i> </i><i>Grilling samples was conducted in
an electric combi oven.</i><i></i></p>
ER -