TY - DATA
T1 - Data underlying the publication: MOSAIC: a highly efficient, one-step recombineering approach to plasmid editing and diversification
PY - 2024/03/14
AU - Marijn van den Brink
AU - Timotheus Althuis
AU - Christophe Danelon
AU - Nico Claassens
UR - 
DO - 10.4121/4464ab86-9214-49b3-a808-10ca655385a6.v1
KW - plasmid libraries
KW - recombineering
KW - MAGE
KW - multiplex
KW - combinatorial libraries
KW - Nanopore sequencing
N2 - <p>Data underlying the publication: [enter citation here].</p><p><br></p><p>This dataset contains data collected during experiments as part of Marijn van den Brink's and Timotheus Y. Althuis' PhD projects. The raw data consists of Nanopore sequencing data. Nanopore sequencing was performed by Plasmidsaurus (Oregon, US). The FASTQ files were then mapped to reference sequences, resulting in .bam/.bai files. These were further analyzed using an in-house developed R script. All data processing and analysis steps are described in detail in the methods section of the publication.</p><p><br></p><p>The data is grouped in folders based on the figures in the publication. The folder name specifies the figure number and contains a short description of the data. Each folder contains raw data (FASTQ) and processed data. The processed data consists of (i) the mapped reads (file format: .bam and .bai), (ii) the analysis results of the mapped reads (file format: .csv and .xlsx), (iii) the graphs plotting the results (file format: .pzfx), and (iv) the R scripts used to produce the results incl. the required .xlsx files.</p>
ER -