TY - DATA
T1 - Data underlying the publication: Model-based aberration corrected microscopy inside a glass tube
PY - 2023/11/29
AU - Daniël Cox
AU - Ivo Vellekoop
UR - 
DO - 10.4121/118c6472-dfc4-419b-ba0f-5d2baba77748.v1
KW - microscopy
KW - adaptive optics
KW - model-based wavefront shaping
KW - aberration correction
KW - digital twin
KW - ray tracing
N2 - <p>Data for the paper "Model-based aberration corrected microscopy inside a glass tube".</p><p><br></p><p>Microscope objectives achieve near diffraction-limited performance only when used under the conditions they are designed for. In non-standard geometries, such as thick cover slips or curved surfaces, severe aberrations arise, inevitably impairing high-resolution imaging. Correcting such large aberrations using standard adaptive optics can be challenging: existing solutions are either not suited for strong aberrations, or require extensive feedback measurements, consequently taking a significant portion of the photon budget. We demonstrate that it is possible to pre-compute the corrections needed for high-resolution imaging inside a glass tube based on a priori information only. Our ray-tracing based method achieved over an order of magnitude increase in image contrast without the need for a feedback signal.</p><p><br></p><p>Contents:</p><p>1. 3-D 2PEF scans of fluorescent beads inside a glass tube.</p><p>2. Brightfield microscopy images of the glass tube.</p><p>3. Parameter scans of the phase correction patterns.</p><p>4. Sensorless AO scans (Zernike mode scans).</p><p>5. Ray-traced model-based phase correction patterns for glass tube.</p><p>6. Code to recreate figures from the paper from raw data.</p><p>7. Protocol to create tube samples.</p>
ER -