TY - DATA
T1 - Data underlying the publication: Mapping recombination landscape and basidial spore number in the button mushroom Agaricus bisporus
PY - 2021/05/28
AU - Brian Lavrijssen
AU - Narges Sedaghat Telgerd
AU - Anton S.M. Sonnenberg
AU - Patrick Hendrickx
UR - https://data.4tu.nl/articles/dataset/Data_underlying_the_publication_Mapping_recombination_landscape_and_basidial_spore_number_in_the_button_mushroom_Agaricus_bisporus/14695425/1
DO - 10.4121/14695425.v1
KW - Basidial spore number (BSN)
KW - button mushroom
KW - QTL
KW - QTL mapping
KW - genotype
KW - recombination landscape
KW - linkage map
KW - intervariatal hybrid
KW - Agaricus bisporus
KW - crossover positions
N2 - - Raw data of counts of basidial spore numbers in lamellae of 2 varieties of A. bisporus, i.e. var. bisporus and var. burnettii (parents).<br>The intervarietal hybrid and the intercross of haploid offspring of the intervarietal hybrid. These data sets are used to map QTL of the basidial spore number.<br>- A selection of 71 haploid offspring of HBT03 that was outcrossed with H39. This set was used to generate 40-50 haploid offspring that was subsequently genotyped to assess crossover positions.<br>These data were used to QTL map the trait " recombination landscape"<br>- Genotypes of the intervarietal hybrid HBT03 (var. bisporus H97 x var. burnettii H119p4).<br>180 homokaryotic single spore isolates (SSIs) were genotyped using 215 SNP's using Kasp genotyping method.<br>- 71 haploid offspring of the intervarietal hybrid HBT03 were outcrossed with the var. bisporis homokaryon H39.<br>From each hybrid, 40-50 haploid offspring was genotyped using 4 SNP markers per chromosome:<br>markers at the chromosome ends, and 2 markers 150-200 kb from chromosome ends ("border" marker).<br><br>
ER -