%0 Generic %A Tschirpke, Sophie %A Laan, Liedewij %A van Opstal, Frank %A van der Valk, Ramon %A Daalman, Werner %D 2023 %T Data underlying the publication: A guide to the in vitro reconstitution of Cdc42 GTPase activity and its regulation %U %R 10.4121/ddc72065-1082-4183-908d-a55b04d05cb2.v1 %K Cdc42 %K gtpase activity %K Cdc42-Cdc24 %K GEF %K cell division %K eukaryotes %K in vitro %X <p>We investigated the<em> in vitro </em>GTPase activity of the <em>S.cerevisae</em> GTPase Cdc42, alone and in combination with its GDP/GTP exchange factor (GEF) Cdc24. We used the GTPase assay 'GTPase-Glo assay' by Promega. The data consists of assay data (amount of remaining GTP for various time points and protein concentrations) for variously tagged Cdc42 constructs and purification batches.</p> %I 4TU.ResearchData