%0 Generic
%A Daran, Jean-Marc
%A Wrońska, Anna
%D 2022
%T Data underlying the study on Engineering class-B vitamin biosynthesis in Saccharomyces cerevisiae
%U https://data.4tu.nl/articles/dataset/Data_underlying_the_study_on_Engineering_class-B_vitamin_biosynthesis_in_Saccharomyces_cerevisiae/17185607/1
%R 10.4121/17185607.v1
%K Saccharomyces cereviseae
%K class B vitamins
%K bradytrophy
%K nutritional requirement
%X <p><i>Saccharomyces cerevisiae</i> is bradytroph for
class B vitamins, it means that yeast cells exhibit slower growth in the absence of an external source of
these metabolites.
Alleviating these nutritional requirements for optimal growth performance would
represent a valuable phenotypic characteristic for industrial strains since
this would result in cheaper processes that would also be less susceptible to
contaminations. In the present study,
suboptimal growth of <i>S. cerevisiae</i> in
absence of either pantothenic acid, <i>para</i>-aminobenzoic acid (<i>p</i>ABA),
pyridoxine, inositol and biotin were corrected by single or double gene
overexpression of native <i>FMS1</i>, <i>ABZ1</i>/<i>ABZ2</i>,
<i>SNZ1</i>/<i>SNO1</i>, <i>INO1</i> and the <i>Cyberlindnera fabianii BIO1</i>, respectively. Several strategies were
attempted to improve growth of <i>S. cerevisiae</i>
CEN.PK113-7D in absence of thiamine, revealing that overexpression of <i>THI4</i> and <i>THI4</i>/<i>THI5</i> was able to
improve growth up to 83% of the maximum specific growth rate of the reference
CEN.PK113-7D in medium including all vitamins. Although the initial aim of this
study was to combine all identified mutations in a single strain, the
engineered strain IMX2210 only harboured genes to correct biotin, <i>p</i>ABA,
pantothenate and inositol bradotrophies. Firstly, this
strain was fast-growing at a maximum specific growth rate of 0.28 ±
0.01 h<sup>-1</sup> in medium devoid of all vitamins. Secondly, this
strain exhibited physiological variables in aerobic glucose limited chemostat
cultures at a dilution rate of 0.1 h<sup>-1</sup> in absence of vitamins
similar to that of the reference strain CEN.PK113-7D grown in the same
conditions but in a fully supplemented complete medium. These physiological
similarities were further emphasized by the limited differences observed in
comparative transcriptome analysis from the chemostat culture grown cells that
were essentially affecting genes of the class B vitamins biosynthetic pathways.
This work paves the way towards construction of the first fast growing
vitamin-independent <i>S. cerevisiae</i>
strain.</p>
%I 4TU.ResearchData