%0 Generic
%A Stallinga, S. (Sjoerd)
%A Liu, Yiming
%A Panezai, Spozmai
%A Wang, Yutong
%D 2024
%T Data underlying the publication: Noise amplification and ill-convergence of Richardson-Lucy deconvolution
%U 
%R 10.4121/b47dff09-f266-42fc-87af-3c7ad9349de7.v1
%K imaging
%K microscopy
%K deconvolution
%K Richardson-Lucy
%K noise
%X <p>The dataset pertains to a study on noise amplification and sensitivity for Richardson-Lucy deconvolution of widefield fluorescence microscopy image data. The image data pertains to the MitoTracker red channel (emission wavelength 599 nm) and&nbsp;Alexa Fluor 488 Phalloidin channel (emission wavelength 512 nm), labeling mitochondria and actin, respectively, of a sample of Bovine Pulmonary Arterial Endothelial cells (BPAE-cells, ThermoFisher FluoCells \#1), imaged with a Nikon Ti-E microscope body with a 40X/NA0.95 objective lens with 1.5X magnification adapter, equipped with an sCMOS camera (Teledyne Kinetix, 3200 3200 pixels, pixel size 6.45 micron. In addition, a set of dark images was recorded for the assessment of offset and readout noise, and a through-focus bead measurement for PSF/OTF assessment.</p>
%I 4TU.ResearchData