%0 Generic
%A Bianchi, Frans
%A Heeringa, Peter
%A Frans, Myrthe
%A van der Veen, Anna
%A van der Ven, Jildau
%D 2022
%T Data underlying the research of the Role of bacterial proteins in the onset of ANCA vasculitis
%U https://data.4tu.nl/articles/dataset/Data_underlying_the_research_of_the_Role_of_bacterial_proteins_in_the_onset_of_ANCA_vasculitis/17171069/1
%R 10.4121/17171069.v1
%K MPO
%K SPIN
%K ANCA
%K AAV
%K vasculitis
%K Anti-neutrophil cytoplasmic antibodies
%K Myeloperoxidase
%K 6pgd
%X Goal: The aim of the project was to investigate whether S.aureus proteins SPIN and 6PGD via the formation of the hybrid auto-antigen SPIN:MPO or MPO mimitope 6PGD could explain the differential aetiologies of the disease GPA (Granulomatosis with polyangitis) caused by MPO-ANCA (Anti Neutrophil Cytoplasmic Autoantibodies).Methods: Isolation kinetic verification of S.Aureus 6PGD and SPIN by means of Activity measurements, see Readme for further specifications. Determination of antibody reactivity/titers using ELISA in sera from patients with MPO ANCA and healthy individuals against the hybrid auto-antigen MPO:ANCA or 6PGD, see Readme for further specifications. Data: Antibody titers ELISA and (Kinetics) colorigenic activity assays.<br>
%I 4TU.ResearchData