%0 Generic
%A Spraggins, Jeffrey M.
%A Van Ardenne, Jacqueline M.
%D 2024
%T Data underlying the publication: Preserving Full Spectrum Information in Imaging Mass Spectrometry Data Reduction
%U 
%R 10.4121/a6efd47a-b4ec-493e-a742-70e8a369f788.v1
%K imaging mass spectrometry
%K MALDI
%K Matrix Completion
%K Full Profile
%K Randomized Matrix Factorization
%X <p><strong>FT-ICR</strong></p><p>Human kidney tissue was surgically removed during a full</p><p>nephrectomy, and remnant tissue was processed for research</p><p>purposes by the Cooperative Human Tissue Network (CHTN)</p><p>at Vanderbilt University Medical Center. Human biospecimens</p><p>were collected in compliance with the CHTN protocols,</p><p>institutional IRB policies, and the National Cancer Institute’s</p><p>best practices for procurement of remnant surgical research</p><p>material. The tissue was flash-frozen over an isopentane-dry</p><p>ice slurry and embedded in carboxymethylcellulose. Tissue</p><p>sections were cryosectioned with a thickness of 10 μm and</p><p>thaw-mounted onto indium tin-oxide-coated glass slides (Delta</p><p>Technologies, Loveland, CO, USA). 1,5-Diaminonaphthalene</p><p>(DAN) was applied to the tissue surface using a TM Sprayer M3</p><p>(HTX Technologies, Chapel Hill, NC, USA). The sample was</p><p>imaged (50 μm pitch) directly after matrix application with</p><p>a 15T MALDI Fourier-transform ion cyclotron resonance (FT-</p><p>ICR) mass spectrometer (Solarix, Bruker Daltonics, Billerica,</p><p>MD, USA). Briefly, data were generated from m/z 300-2000</p><p>with a 4M file size in negative ion mode.</p><p><br></p><p><strong>qTOF</strong></p><p>C57BL6/J mice were retro-orbitally infected with <em>S. aureus</em> Newman and sacrificed humanely five days post-infection. Kidneys were flash frozen on an isopentane/dry ice slurry and embedded in 2.6\% carboxymethylcellulose. 5 μm thick sections were collected using a Leica Biosystems CM3050S cryostat and thaw-mounted onto indium tin oxide coated glass slides. Sections were washed with cold 150 mM ammonium formate for 45 seconds for three total washes. 5 mg of 4-(dimethylamino)cinnamic acid matrix was applied using an in-house sublimation device. MALDI IMS data were collected in negative ion mode from m/z 400-2000 using a Bruker MALDI timsTOF fleX platform with a 5 μm step size, 25% laser power, and 25 shots per pixel.</p>
%I 4TU.ResearchData