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Background: The sulfur oxygen reductase peroxidase (TPx) protein from Cellulosimicrobium cellulans excretory-secretory antigen (ESA) has been shown to regulate the differentiation of host Treg and Th17 cells, resulting in an immune suppression response dominated by Treg cells. However, the molecular mechanisms by which the TPx protein from Cellulosimicrobium cellulans ESA regulates the differentiation of host Treg/Th17 cells have not been reported.
Methods: Jurkat cells stimulated with TPx protein from porcine Cellulosimicrobium cellulans ESAs at 0, 24, 48, and 72 hours with PMA and ionomycin activation. Transcriptomic analysis was performed to study the signaling pathways related to Jurkat cell differentiation regulated by TPx protein from porcine Cellulosimicrobium cellulans ESA.
Results: Gene Set Enrichment Analysis (GSEA) showed that TPx protein from porcine Cellulosimicrobium cellulans ESAs can induce upregulation of the TGF-β signaling pathway in Jurkat cells and downregulation of Th17 cell differentiation.
Conclusion: The TPx protein from porcine Cellulosimicrobium cellulans ESA can activate the TGF-β signaling pathway in Jurkat cells, thereby regulating the differentiation of Treg/Th17 cells, resulting in an immune suppression response dominated by Treg cells and evading host immune attacks. This study provides a foundation for further validating these pathways and elucidating the molecular mechanisms of immune escape caused by porcine Cellulosimicrobium cellulans.
Original text:
背景：来自纤维素囊尾蚴（C. cellulosae）排泄分泌抗原（ESA）的硫氧还原蛋白过氧化物酶（TPx）蛋白已被证明可以调节宿主Treg和Th17细胞的分化，导致由Treg细胞主导的免疫抑制反应。然而，来自纤维素梭菌ESA的TPx蛋白调节宿主Treg/Th17细胞分化失衡的分子机制尚未报道。（2）方法：采用猪纤维素梭菌ESAs的TPx蛋白在第0、24、48和72小时刺激PMA和电离霉素活化的Jurkat细胞。进行转录组学分析以研究与来自纤维素梭菌ESA的TPx蛋白调控的Jurkat细胞分化相关的信号通路。（3）结果：基因集富集分析（GSEA）显示，来自猪纤维素梭菌ESAs的TPx蛋白可诱导Jurkat细胞TGF-β信号通路上调和Th17细胞分化下调。（4）结论：猪纤维素梭菌ESA的TPx蛋白可激活Jurkat细胞中的TGF-β信号通路，从而调节Treg/Th17细胞的分化，导致以Treg细胞为主的免疫抑制反应，从而逃避宿主免疫攻击。本研究为进一步验证这些途径提供了基础，并进一步阐明了猪纤维素梭菌引起的免疫逃逸的分子机制。