%0 Generic %A Lavrijssen, Brian %A Telgerd, Narges Sedaghat %A Sonnenberg, Anton S.M. %A Hendrickx, Patrick %D 2021 %T Data underlying the publication: Mapping recombination landscape and basidial spore number in the button mushroom Agaricus bisporus %U https://data.4tu.nl/articles/dataset/Data_underlying_the_publication_Mapping_recombination_landscape_and_basidial_spore_number_in_the_button_mushroom_Agaricus_bisporus/14695425/2 %R 10.4121/14695425.v2 %K Basidial spore number (BSN) %K button mushroom %K QTL %K QTL mapping %K genotype %K recombination landscape %K linkage map %K intervariatal hybrid %K Agaricus bisporus %K crossover positions %X - Raw data of counts of basidial spore numbers in lamellae of 2 varieties of A. bisporus, i.e. var. bisporus and var. burnettii (parents).<br>The intervarietal hybrid and the intercross of haploid offspring of the intervarietal hybrid. These data sets are used to map QTL of the basidial spore number.<br>- A selection of 71 haploid offspring of HBT03 that was outcrossed with H39. This set was used to generate 40-50 haploid offspring that was subsequently genotyped to assess crossover positions.<br>These data were used to QTL map the trait " recombination landscape"<br>- Genotypes of the intervarietal hybrid HBT03 (var. bisporus H97 x var. burnettii H119p4).<br>180 homokaryotic single spore isolates (SSIs) were genotyped using 215 SNP's using Kasp genotyping method.<br>- 71 haploid offspring of the intervarietal hybrid HBT03 were outcrossed with the var. bisporis homokaryon H39.<br>From each hybrid, 40-50 haploid offspring was genotyped using 4 SNP markers per chromosome:<br>markers at the chromosome ends, and 2 markers 150-200 kb from chromosome ends ("border" marker).<br><br> %I 4TU.ResearchData