cff-version: 1.2.0
abstract: "<p>Engineering a new metabolic function in a microbial host can
be limited by the availability of the relevant co-factor. For instance, in <i>Yarrowia lipolytica</i>, the expression of a
functional nitrate reductase is
precluded by the absence of molybdenum cofactor (Moco) biosynthesis. In this study, we demonstrated
that the <i>Ogataea parapolymorpha</i> Moco
biosynthesis pathway associated with the expression of a high affinity
molybdate transporter could lead to the synthesis of Moco in <i>Y. lipolytica</i>. This was achieved by
coupling Moco biosynthesis to the Moco-dependent nitrate assimilation pathway
of the same donor, <i>O. parapolymorpha</i>. In addition to 11 heterologous genes,
fast growth on nitrate required adaptive laboratory evolution which, resulted
in up to 100-fold increase in nitrate reductase activity and in up to 4-fold
increase in growth rate. Genome sequencing of evolved isolates revealed the
presence of a limited number of non-synonymous mutations or small
insertions/deletions in annotated coding sequences. This study that builds up
on a previous work establishing Moco synthesis in <i>S. cerevisiae</i> demonstrated that the Moco pathway could be
successfully transferred in very distant yeasts and, potentially, to any other genera,
which would enable the expression of new enzyme families and expand the nutrient
range used by industrial yeasts.</p>"
authors:
  - family-names: Daran
    given-names: Jean-Marc
    orcid: "https://orcid.org/0000-0003-3136-8193"
  - family-names: Perli
    given-names: Thomas
title: "Data underlying research on Engineering of molybdenum-cofactor-dependent nitrate assimilation in Yarrowia lipolytica"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/14230238.v1
license: CC BY-NC 4.0
date-released: 2021-03-18