cff-version: 1.2.0
abstract: "The synthesis of N-acetylneuraminic acid (Neu5Ac) derivatives is drawing more and more attention in glycobiology research because of the important role of sialic acids in e. g. cancer, bacterial, and healthy cells. Chemical preparation of these carbohydrates typically relies on multistep synthetic procedures leading to low overall yields. Herein we report a continuous flow process involving N-acetylneuraminate lyase (NAL) immobilized on Immobead 150P (Immobead-NAL) to prepare Neu5Ac derivatives. Batch experiments with Immobead-NAL showed equal activity as the native enzyme. Moreover, by using a fivefold excess of either N-acetyl-D-mannosamine (ManNAc) or pyruvate the conversion and isolated yield of Neu5Ac were significantly improved. To further increase the efficiency of the process, a flow setup was designed providing a chemoenzymatic entry into a series of N-functionalized Neu5Ac derivatives in conversions of 48–82%, and showing excellent stability over 1 week of continuous use."
authors:
  - family-names: Bloemendal
    given-names: Victor
    orcid: "https://orcid.org/0000-0003-0013-6015"
  - family-names: Moons
    given-names: S.J. (Sam)
    orcid: "https://orcid.org/0000-0001-5507-3811"
  - family-names: Heming
    given-names: J.J.A. (Jurriaan)
  - family-names: Chayoua
    given-names: M. (Mohamed)
  - family-names: Niesink
    given-names: O. (Olaf)
  - family-names: van Hest
    given-names: Jan
    orcid: "https://orcid.org/0000-0001-7973-2404"
  - family-names: Boltje
    given-names: T.J. (Thomas)
    orcid: "https://orcid.org/0000-0001-9141-8784"
  - family-names: Rutjes
    given-names: F.P.J.T. (Floris)
    orcid: "https://orcid.org/0000-0003-1538-3852"
title: "Data underlying the article: Chemoenzymatic Synthesis of Sialic Acid Derivatives Using Immobilized N-Acetylneuraminate Lyase in a Continuous Flow Reactor"
keywords:
version: 1
identifiers:
  - type: doi
    value: 10.4121/uuid:4c72b586-a07a-484e-9cb4-5ea4b3082962
license: CC0
date-released: 2019-10-14