Data underlying the publication: "Mechanosensitive interactions between Jag1 and Myo1c control Jag1 trafficking in endothelial cells"

DOI:10.4121/d7111159-ca2c-430c-8c29-10ba2a6ca8d7.v2
The DOI displayed above is for this specific version of this dataset, which is currently the latest. Newer versions may be published in the future. For a link that will always point to the latest version, please use
DOI: 10.4121/d7111159-ca2c-430c-8c29-10ba2a6ca8d7

Datacite citation style

Stassen, O. M. J. A. (Oscar); Virtanen, Noora; Suarez Rodriguez, F. (Freddy); Lin, Kai-Lan; Heijmans, M.J.M. (Matthijs) et. al. (2025): Data underlying the publication: "Mechanosensitive interactions between Jag1 and Myo1c control Jag1 trafficking in endothelial cells". Version 2. 4TU.ResearchData. dataset. https://doi.org/10.4121/d7111159-ca2c-430c-8c29-10ba2a6ca8d7.v2
Other citation styles (APA, Harvard, MLA, Vancouver, Chicago, IEEE) available at Datacite

Dataset

Version 2 - 2025-10-15 (latest)
Version 1 - 2025-10-06

Morphogenesis of the cardiovascular system is responsive to hemodynamic cues sensed by endothelial cells. The organization of morphogenic signaling proteins is regulated by membrane presentation and internalization. Here we aimed to characterize factors that regulate this flow dependent protein localization by identifying differential interactors with the Notch ligand Jagged1 in response to shear stress. We cultured endothelial cells expressing Jagged1-APEX2 for proximity labeling on an orbital shaker shear stress platform. Myo1c was identified and confirmed through coimmunoprecipitation as a Jagged1 interacting factor under static conditions, with reduced interaction after exposure to shear. Jagged1 polarization downstream of shear followed by nucleograde transport was inhibited by Myo1c knockout. Further, Myo1c knockdown reduced membrane levels of Jagged1 under static, but not shear conditions. Together, our data reveal a role for Myo1c in the hemodynamic control of Jagged1 localization in endothelial cells.


This dataset contains data from in vitro experiments studying the interaction between Myo1c and Jagged1 under fluid flow shear stress.

History

  • 2025-10-06 first online
  • 2025-10-15 published, posted

Publisher

4TU.ResearchData

Format

spreadsheet/.csv spreadsheet/.xlsx image/.png image/.tif westernblot/.g2i

Funding

  • MECHANOREGULATION OF JAGGED IN VASCULAR TISSUE HOMEOSTASIS (grant code 307133) [more info...] Academy of Finland
  • The integration of cell signalling and mechanical forces in vascular morphology (grant code 771168) [more info...] European Research Council
  • Spatiotemporal Control of Cell Functions (grant code 316882) [more info...] Academy of Finland
  • Multilayer mechanosignalling in vascular homeostasis-SIGNALSHEETS (grant code 330411) [more info...] Academy of Finland
  • Strategic research profiling area Solutions for Health at Åbo Akademi University (grant code 336355) Academy of Finland
  • InFLAMES: Innovation Ecosystem based on the Immune System (grant code 337531) [more info...] Academy of Finland
  • Tumor Lymph Node on Chip (grant code 953234) European Union Research Council

Organizations

Åbo Akademi University, Faculty of Science and Engineering, Cell Biology, Turku, Finland
Åbo Akademi University and University of Turku, Turku Bioscience, Turku, Finland
Åbo Akademi University and University of Turku, InFLAMES Research Flagship Center, Turku, Finland
TU Eindhoven, Department of Biomedical Engineering, Eindhoven, the Netherlands
TU Eindhoven, Institute for Complex Molecular Systems (ICMS), the Netherlands
Swansea University, Faculty of Science and Engineering, Department of Biomedical Engineering, Zienkiewicz Institute for Modelling, Data & AI, Swansea, United Kingdom
University of Amsterdam, Van ‘t Hoff Institute for Molecular Sciences (HIMS), , Amsterdam, the Netherlands

DATA

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