1/1
16 files

Fluorescence microscopy, image analysis and quantitative PCR data underlying the publication “Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting”

dataset
posted on 06.05.2022, 07:22 by Laura A. Tiemeijer, Tommaso RistoriTommaso Ristori, O. M. J. A. (Oscar) Stassen, Jaakko Ahlberg, Jonne J. J. de Bijl, Christopher S. Chen, Katie BentleyKatie Bentley, C. V. C (Carlijn) Bouten, C. M. (Cecilia) Sahlgren

Spatial regulation of angiogenesis is important for the generation of functional engineered vasculature and the Notch ligands Jag1 and Dll4 are known to regulate endothelial sprouting. In this project, in vitro and in silico models were used to analyze the ability of micropatterned Jag1 and Dll4 to spatially control the sprouting process. Dll4 patterns, but not Jag1 patterns, were found to elicit spatial control and computational simulations suggest different timing of Notch activation by Jag1 and Dll4 to be the reason for their distinct effects. The more potent spatial control of sprouting by Dll4 in comparison to Jag1 could be exploited as a tool in vascular engineering and regenerative medicine.


This dataset contains data from in vitro experiments on endothelial sprouting with human endothelial cells and micropatterned Jag1 and Dll4 ligands. 

Funding

MECHANOREGULATION OF JAGGED IN VASCULAR TISSUE HOMEOSTASIS

Academy of Finland

Find out more...

Spatiotemporal Control of Cell Functions

Academy of Finland

Find out more...

Multilayer mechanosignalling in vascular homeostasis-SIGNALSHEETS

Academy of Finland

Find out more...

Investigating the crosstalk between Notch and YAP/TAZ in sprouting angiogenesis

European Commission

Find out more...

The integration of cell signalling and mechanical forces in vascular morphology

European Research Council

Find out more...

Doctoral Network in Molecular Biosciences at Åbo Akademi University

Cellular Adaptive Behaviour Laboratory

Cancer Research UK

Find out more...

Materials-driven regeneration: Regenerating tissue and organ function with intelligent, life-like materials

Netherlands Organisation for Scientific Research

Find out more...

InFLAMES: Innovation Ecosystem based on the Immune System

Academy of Finland

Find out more...

Åbo Akademi University Foundation’s Center of Excellence in Cellular Mechanostasis

History

Publisher

4TU.ResearchData

Format

directionality histograms (.png) directionality orientation maps (.tif) directionality analysis (.csv) fluorescence microscopy images (.tif) gene expression analysis, qPCR data (.txt) raw microscopy files (.lif) sprouting efficiency ROIs (.tif) sprouting efficiency analysis (.csv)

Organizations

Faculty for Science and Engineering, Biosciences, Åbo Akademi University, Turku,Finland Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, Eindhoven, The Netherlands The Biological Design Center and Department of Biomedical Engineering, Boston University, Boston, USA Turku Bioscience Centre, Åbo Akademi University and University of Turku, Finland The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, USA The Francis Crick Institute, London, UK Department of Informatics, King’s College London, UK