Data underlying the research on particle manipulation using hydrodynamic forcing. This dataset consist of the experimental data of an arbitrarily selected pollen grain trapping in a Hele-Shaw cell.
The reseach objective is to present a microfluidic approach to achieve the dynamic control of particle pathlines within a flow through microfluidic device. Our approach is general, and not limited to one particular kind of particle. The flow is used as gentle tweezers to move particles within the flow cell and the approach is particularly suited to biological sample. To demonstrate this, an arbitrary pollen grain is selected and trapped in the flow cell while the other pollen grains continue to flow. Our approach combines three key aspects: the design of a flow-through microfluidic flow cell with the ability to manipulate the streamlines of the flow, an optimization procedure to find a priori optimal particle path-lines, and a Proportion-Integral-Derivative-based (PID) feedback controller to provide real time control over the particle manipulations. The experimental raw images were recorded with a sCMOS camera (PCO) with a pixel pitch of 6.5 μm. The camera was mounted on a microscope (Nikon Eclipse Ti) with a 1x objective. The acquisition frequency was 5 Hz corresponding to an average in-plane displacement of 4-6 pixels between two consecutive recordings. An additional sCMOS camera (PCO) with 3x zoom lens is used for the visualization of the trapped pollen. The zip file contains the raw images and the MATLAB script used to do an experiment of arbitarily selecting a pollen grain and trapping it by only using the hydrodynamic forcing in a Hele-Shaw cell.