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Data underlying the research of Chemoenzymatic Synthesis of Sialic Acid Derivatives Using Immobilized N-Acetylneuraminate Lyase in a Continuous Flow Reactor

posted on 25.04.2019, 00:00 authored by Victor BloemendalVictor Bloemendal, S.J. (Sam) Moons, J.J.A. (Jurriaan) Hemming, M. (Mohamed) Chayoua, O. (Olaf) Niesink, Jan van HestJan van Hest, T.J. (Thomas) Boltje, F.P.J.T. (Floris) Rutjes
NMR research data related to a continuous flow process involving N‐acetylneuraminate lyase (NAL) immobilized on Immobead 150P to prepare Neu5Ac derivatives. Batch experiments with Immobead‐NAL showed equal activity as the native enzyme. By using a fivefold excess of either N‐acetyl‐D‐mannosamine (ManNAc) or pyruvate the conversion and isolated yield of Neu5Ac were significantly improved. To further increase the efficiency of the process, a flow setup was designed providing a chemoenzymatic entry into a series of N‐functionalized Neu5Ac derivatives in conversions of 48‐82%, and showing excellent stability over 1 week of continuous use.



Eindhoven University of Technology; Radboud University Nijmegen


4TU.Centre for Research Data


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