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Data underlying the research of Chemoenzymatic Synthesis of Sialic Acid Derivatives Using Immobilized N-Acetylneuraminate Lyase in a Continuous Flow Reactor

dataset
posted on 25.04.2019 by Victor Bloemendal, S.J. (Sam) Moons, J.J.A. (Jurriaan) Hemming, M. (Mohamed) Chayoua, O. (Olaf) Niesink, Jan van Hest, T.J. (Thomas) Boltje, F.P.J.T. (Floris) Rutjes
NMR research data related to a continuous flow process involving N‐acetylneuraminate lyase (NAL) immobilized on Immobead 150P to prepare Neu5Ac derivatives. Batch experiments with Immobead‐NAL showed equal activity as the native enzyme. By using a fivefold excess of either N‐acetyl‐D‐mannosamine (ManNAc) or pyruvate the conversion and isolated yield of Neu5Ac were significantly improved. To further increase the efficiency of the process, a flow setup was designed providing a chemoenzymatic entry into a series of N‐functionalized Neu5Ac derivatives in conversions of 48‐82%, and showing excellent stability over 1 week of continuous use.

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Contributors

Eindhoven University of Technology; Radboud University Nijmegen

Publisher

4TU.Centre for Research Data

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media types: application/octet-stream, application/pdf, application/x-rar-compressed, text/plain, text/xml

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