Data underlying the publication: Development of high frequency in vitro shoot regeneration system from leaves of apple cultivar Oregon Spur and optimization of antibiotics concentration
the present study is carried out optimize the high efficiency shoot regeneration protocol from the leaf explant of the Oregon Spur cultivar. The different concentration of the Cytokinin (BAP, TDZ) and auxin (NAA, IBA) were tested to identify the optimium ratio and quantity required to generate shoots. 4mg/l BA alongwith 0.2mg/l NAA was found more effective cytokinin than TDZ for high quality shoot induction. Effect of dark treatment was also tested on the shoot regeneration and inhbitory effect was observed. During optimization of the antibiotics concentration, Leaf explant were found highly sensitive to Kanamycin and inhibited shoot induction above 5mg/l, whereas cefotaxime at 100-300mg/l promoted organogenesis. 500mg/l cefotaxime was found optimal to eliminate bacterial overgrowth after co-cultivation and 5 mg/l of kanamycin was found best for selection of plant.